PSMD6 Mouse Monoclonal Antibody [A9E4]
Catalog# HA601127
PSMD6 Mouse Monoclonal Antibody [A9E4]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
PSMD6 Mouse Monoclonal Antibody [A9E4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human PSMD6 aa 1-150 / 389.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 45.5 kDa
Positive Control
K562 cell lysate, MCF-7 cell lysate, HEK-293 cell lysate, Hela cell lysate, THP-1 cell lysate, human kidney tissue lysate, human testis tissue.
Conjugation
unconjugated
Clone Number
A9E4
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
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| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IHC-P
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1:200
Target
Function
This gene encodes a member of the protease subunit S10 family. The encoded protein is a subunit of the 26S proteasome which colocalizes with DNA damage foci and is involved in the ATP-dependent degradation of ubiquinated proteins. Alternative splicing results in multiple transcript variants. Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.
Background References
1. Chen M et al. A variant of PSMD6 is associated with the therapeutic efficacy of oral antidiabetic drugs in Chinese type 2 diabetes patients. Sci Rep. 2015 May
Subcellular Location
Cytosol, extracellular region, nucleoplasm.
UNIPROT
Synonyms
26S proteasome non-ATPase regulatory subunit 6 antibody
26S proteasome regulatory subunit RPN7 antibody
26S proteasome regulatory subunit S10 antibody
Breast cancer-associated protein SGA-113M antibody
KIAA0107 antibody
p42A antibody
P44S10 antibody
PFAAP4 antibody
Phosphonoformate immuno-associated protein 4 antibody
Proteasome (prosome, macropain) 26S subunit, non-ATPase, 6 antibody
ExpandImages
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Western blot analysis of PSMD6 on different lysates with Mouse anti-PSMD6 antibody (HA601127) at 1/1,000 dilution.
Lane 1: K562 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: Hela cell lysate
Lane 5: THP-1 cell lysate
Lane 6: Human kidney tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 45.5 kDa
Observed band size: 40 kDa
Exposure time: 1 minute;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601127) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-PSMD6 antibody (HA601127) at 5μg/mL dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601127) at 5μg/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"