Cyclin E2 Recombinant Mouse Monoclonal Antibody [40-89-R] (HA601146)
Catalog# HA601146
Mouse Monoclonal to Cyclin E2
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WB
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IHC-P
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IF-Cell
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Human
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K-562 cell lysate
Lane 4: A549 cell lysate
Lane 5: MCF7 cell lysate
Lane 6: HEK-293 cell lysate
Lane 7: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 51/47 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601146) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Mouse monoclonal primary
Product Name
Cyclin E2 Recombinant Mouse Monoclonal Antibody [40-89-R] (HA601146)
Immunogen
Synthetic peptide within human cyclin e2 aa 1-50 / 404.
Host
Mouse
Positive Control
HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, A549 cell lysate, MCF7 cell lysate, HEK-293 cell lysate, HepG2 cell lysate, human thyroid tissue, human testis tissue, HeLa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
40-89-R
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:200-1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 47 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cyclin E2
SYNONYMS
CCN E2 antibody;CCNE 2 antibody;CCNE2 antibody;CCNE2 protein antibody;CYC E2 antibody;CYCE 2 antibody;CYCE2 antibody;CyclinE2 antibody;G1/S specific cyclin E2 antibody
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Cyclin E2 is a protein that in humans is encoded by the CCNE2 gene. It is a G1 cyclin that binds Cdk2 and is inhibited by p27(Kip1) and p21(Cip1). It plays a role in the G1/S portion of the cell cycle and also has putative interactions with proteins CDKN1A, CDKN1B, and CDK3. Aberrant expression can lead to cancer.
CITATIONS
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Chinese Propolis Inhibits the Proliferation of Human Gastric Cancer Cells by Inducing Apoptosis and Cell Cycle Arrest
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WB
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Human
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Citation
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