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GSTO1 Mouse Monoclonal Antibody [A9F11]

Usd: 350

Specification

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Catalog# HA601148

GSTO1 Mouse Monoclonal Antibody [A9F11]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

GSTO1 Mouse Monoclonal Antibody [A9F11]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human GSTO1 aa 1-241/241.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 28 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, U-87 MG cell lysate, A172 cell lysate.

Conjugation

unconjugated

Clone Number

A9F11

RRID

AB_3071870

Reactivity Data

WB
Human
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

Glutathione S-transferase omega-1 is an enzyme that in humans is encoded by the GSTO1 gene. This gene encodes a member of the theta class glutathione S-transferase-like (GSTTL) protein family. In mouse, the encoded protein acts as a small stress response protein, likely involved in cellular redox homeostasis. This protein has dehydroascorbate reductase activity and may function in the glutathione-ascorbate cycle as part of antioxidant metabolism.

Background References

1. Paul S et al. GSTO1 confers drug resistance in HCT‑116 colon cancer cells through an interaction with TNFαIP3/A20. Int J Oncol. 2022 Nov

2. Wang L et al. GSTO1 regulates insulin biosynthesis in pancreatic β cells. Biochem Biophys Res Commun. 2020 Apr

Subcellular Location

Cytoplasm, cytosol.

UNIPROT

SwissProt: P78417 Human

Synonyms

AA407097 antibody

AI194287 antibody

AU018802 antibody

DKFZp686H13163 antibody

EC 2.5.1.18 antibody

Glutathione S transferase omega 1 antibody

Glutathione S-transferase omega 1-1 antibody

Glutathione S-transferase omega antibody

Glutathione S-transferase omega-1 antibody

Glutathione transferase omega 1 antibody

Expand

Images

  • Western blot analysis of GSTO1 on different lysates with Mouse anti-GSTO1 antibody (<a href="/products/HA601148" style="font-weight: bold;text-decoration: underline;">HA601148</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: U-87 MG cell lysate<br />Lane 5: A172 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 28 kDa<br />Observed band size: 28 kDa<br /><br />Exposure time: 58 seconds;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601148" style="font-weight: bold;text-decoration: underline;">HA601148</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:150,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GSTO1 on different lysates with Mouse anti-GSTO1 antibody (HA601148) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: U-87 MG cell lysate
    Lane 5: A172 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

    Exposure time: 58 seconds;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601148) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation