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ACSL5 Recombinant Mouse Monoclonal Antibody [A9D4-R]

Usd: 350

Specification

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Catalog# HA601149

ACSL5 Recombinant Mouse Monoclonal Antibody [A9D4-R]

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

ACSL5 Recombinant Mouse Monoclonal Antibody [A9D4-R]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human aa 151-300 / 683.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 76 kDa

Positive Control

Mouse liver tissue lysate, rat liver tissue lysate, mouse liver tissue, rat liver tissue.

Conjugation

unconjugated

Clone Number

A9D4-R

RRID

AB_3071871

Reactivity Data

WB IHC-P
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Human
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Long-chain-fatty-acid—CoA ligase 5 is an enzyme that in humans is encoded by the ACSL5 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.

Background References

1. Hou T et al. Cytoplasmic SIRT6-mediated ACSL5 deacetylation impedes nonalcoholic fatty liver disease by facilitating hepatic fatty acid oxidation. Mol Cell. 2022 Nov

2. Luo Q et al. Role of ACSL5 in fatty acid metabolism. Heliyon. 2023 Jan

Subcellular Location

Mitochondrion, Endoplasmic reticulum, Mitochondrion outer membrane, Endoplasmic reticulum membrane, Cell membrane.

UNIPROT

SwissProt: Q8JZR0 Mouse

SwissProt: O88813 Rat

Synonyms

ACS2 antibody

ACS5 antibody

Acyl CoA synthetase 5 antibody

Acyl CoA synthetase long chain family member 5 antibody

EC 6.2.1.3 antibody

FACL5 antibody

FACL5 for fatty acid coenzyme A ligase 5 antibody

Fatty acid CoA ligase, long chain 5 antibody

Fatty acid coenzyme A ligase 5 antibody

Fatty acid Coenzyme A ligase long chain 5 antibody

Expand

Images

  • Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (<a href="/products/HA601149" style="font-weight: bold;text-decoration: underline;">HA601149</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse liver tissue lysate<br />Lane 2: Rat liver tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 76 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601149" style="font-weight: bold;text-decoration: underline;">HA601149</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (HA601149) at 1/1,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Rat liver tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 76 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601149) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (<a href="/products/HA601149" style="font-weight: bold;text-decoration: underline;">HA601149</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601149" style="font-weight: bold;text-decoration: underline;">HA601149</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (HA601149) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601149) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-ACSL5 antibody (<a href="/products/HA601149" style="font-weight: bold;text-decoration: underline;">HA601149</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601149" style="font-weight: bold;text-decoration: underline;">HA601149</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-ACSL5 antibody (HA601149) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601149) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"