MVK Mouse Monoclonal Antibody [A9G12]
Catalog# HA601151
MVK Mouse Monoclonal Antibody [A9G12]
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
MVK Mouse Monoclonal Antibody [A9G12]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human 197-396 / 396.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 42.5 kDa
Positive Control
HepG2 cell lysate, Jurkat cell lysate, COS-1 cell lysate, SK-Br-3 cell lysate, HEK-293 cell lysate, K-562 cell lysate, MCF7 cell lysate, A431 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, human kidney tissue, human liver tissue, human testis tissue, Jurkat.
Conjugation
unconjugated
Clone Number
A9G12
RRID
Reactivity Data
| WB | IHC-P | IF-Cell | |
|---|---|---|---|
| Human |
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| Mouse |
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|
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| Rat |
|
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| Green Monkey |
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Cell
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1:100
Target
Function
Mevalonate kinase is an enzyme (specifically a kinase) that in humans is encoded by the MVK gene. Mevalonate kinases are found in a wide variety of organisms from bacteria to mammals. Mevalonate is a key intermediate, and mevalonate kinase a key early enzyme, in isoprenoid and sterol synthesis. As the second enzyme in the Mevalonate pathway, it catalyzes the phosphorylation of Mevalonic acid to produce Mevalonate-5-phosphate. A 5-10% reduction in mevalonate kinase activity is associated with the mevalonate kinase deficiency (MVD) resulting in accumulation of intermediate mevalonic acid.
Background References
1. Valpreda G et al. Dual MVK cleavable linkers effectively reduce renal retention of 111In-fibronectin-binding peptides. Bioorg Med Chem. 2022 Nov
2. Wang J et al. Analysis of MVK gene variant in a child with high IgD syndrome caused by mevalonate kinase deficiency. 2022 Apr
Subcellular Location
Cytoplasm, Peroxisome.
Synonyms
FLJ96772 antibody
KIME_HUMAN antibody
LH receptor mRNA binding protein antibody
LRBP antibody
Mevalonate kinase 1 antibody
Mevalonate kinase antibody
Mevalonic aciduria antibody
MK antibody
mvk antibody
MVLK antibody
ExpandImages
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Western blot analysis of MVK on different lysates with Mouse anti-MVK antibody (HA601151) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: COS-1 cell lysate
Lane 4: SK-Br-3 cell lysate
Lane 5: HEK-293 cell lysate
Lane 6: K-562 cell lysate
Lane 7: MCF7 cell lysate
Lane 8: A431 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42.5 kDa
Observed band size: 42.5/130 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601151) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-MVK antibody (HA601151) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601151) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-MVK antibody (HA601151) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601151) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-MVK antibody (HA601151) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601151) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of Jurkat cells labeling MVK with Mouse anti-MVK antibody (HA601151) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MVK antibody (HA601151) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"