Human IL-3 Mouse Monoclonal Antibody [PSH0-98] - BSA and Azide free (Detector)
Overview
Product Name
Human IL-3 Mouse Monoclonal Antibody [PSH0-98] - BSA and Azide free (Detector)
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant full length human IL-3 protein.
Species Reactivity
Human
Validated Applications
ELISA(Det)
Molecular Weight
Predicted band size: 17.2 kDa
Positive Control
Recombinant human IL-3 protein
Conjugation
unconjugated
Clone Number
PSH0-98
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
1:5,000
Target
Function
Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB. Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program (By similarity). Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK.
Background References
1. Hong L., Tang Y., Pan S., Xu M., Shi Y., Gao S., Sui C., He C., Zheng K., Tang R., Shi Z., Wang Q., Wang H. Interleukin 3-induced GITR promotes the activation of human basophils. Cytokine 136:155268-155268 (2020).
2. Lopez C., Zamorano P., Teuber S., Salas M., Otth C., Hidalgo M.A., Concha I., Zambrano A. Interleukin-3 Prevents Cellular Death Induced by Oxidative Stress in HEK293 Cells. J. Cell. Biochem. 118:1330-1340 (2017).
Subcellular Location
Secreted
UNIPROT
Synonyms
Colony stimulating factor multiple antibody
Hematopoietic growth factor antibody
IL 3 antibody
IL-3 antibody
IL3 antibody
IL3_HUMAN antibody
Interleukin 3 (colony stimulating factor, multiple) antibody
Interleukin 3 antibody
Interleukin-3 antibody
Mast cell growth factor antibody
ExpandImages
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![Standard curve of human IL-3 matched pair antibodies:<br /><br />Sandwich ELISA analysis of human IL-3 matched pair antibodies<br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH0-96] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IL-3 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH0-98]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA601155_1.png?v=20260429161621)
Standard curve of human IL-3 matched pair antibodies:
Sandwich ELISA analysis of human IL-3 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH0-96] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IL-3 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH0-98]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Standard curve of human IL-3 matched pair antibodies:<br /><br />Sandwich ELISA analysis of human IL-3 matched pair antibodies<br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH0-97] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IL-3 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH0-98]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA601155_2.png?v=20260429161621)
Standard curve of human IL-3 matched pair antibodies:
Sandwich ELISA analysis of human IL-3 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH0-97] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IL-3 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH0-98]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"