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SLC39A8 Mouse Monoclonal Antibody [A9G3]

Usd: 350

Specification

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Catalog# HA601161

SLC39A8 Mouse Monoclonal Antibody [A9G3]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

SLC39A8 Mouse Monoclonal Antibody [A9G3]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human SLC39A8 aa 23-132 (Extracellular).

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 49.6 kDa

Positive Control

Jurkat cell lysate, SiHa cell lysate, HeLa cell lysate, HUVEC cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate.

Conjugation

unconjugated

Clone Number

A9G3

RRID

AB_3071880

Reactivity Data

WB
Human
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgM

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Electroneutral divalent metal cation:bicarbonate symporter of the plasma membrane mediating the cellular uptake of zinc and manganese, two divalent metal cations important for development, tissue homeostasis and immunity. Transports an electroneutral complex composed of a divalent metal cation and two bicarbonate anions or alternatively a bicarbonate and a selenite anion. Thereby, it also contributes to the cellular uptake of selenium, an essential trace metal and micronutrient. Also imports cadmium a non-essential metal which is cytotoxic and carcinogenic. May also transport iron and cobalt through membranes. Through zinc import, indirectly regulates the metal-dependent transcription factor MTF1 and the expression of some metalloproteases involved in cartilage catabolism and also probably heart development. Also indirectly regulates the expression of proteins involved in cell morphology and cytoskeleton organization. Indirectly controls innate immune function and inflammatory response by regulating zinc cellular uptake which in turn modulates the expression of genes specific of these processes. Protects, for instance, cells from injury and death at the onset of inflammation. By regulating zinc influx into monocytes also directly modulates their adhesion to endothelial cells and arteries. Reclaims manganese from the bile at the apical membrane of hepatocytes, thereby regulating the activity of the manganese-dependent enzymes through the systemic levels of the nutrient.

Background References

1. Choi E.K., Nguyen T.T., Gupta N., Iwase S., Seo Y.A. Functional analysis of SLC39A8 mutations and their implications for manganese deficiency and mitochondrial disorders. Sci. Rep. 8:3163-3163 (2018).

2. Wang C.Y., Jenkitkasemwong S., Duarte S., Sparkman B.K., Shawki A., Mackenzie B., Knutson M.D. ZIP8 is an iron and zinc transporter whose cell-surface expression is up- regulated by cellular iron loading. J. Biol. Chem. 287:34032-34043 (2012).

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q9C0K1 Human

SwissProt: Q91W10 Mouse

SwissProt: Q5FVQ0 Rat

Synonyms

4933419D20Rik antibody

AA986696 antibody

BCG induced integral membrane protein 103 antibody

BCG induced integral membrane protein BIGMo-103 (Up-regulated by BCG-CWS) (Hypothetical protein) antibody

LIV-1 subfamily of ZIP zinc transporter 6 antibody

LZT Hs6 antibody

MGC108852 antibody

PP3105 antibody

SLC39A8 antibody

Solute carrier family 39 (zinc transporter), member 8 antibody

Expand

Images

  • Western blot analysis of SLC39A8 on different lysates with Mouse anti-SLC39A8 antibody (<a href="/products/HA601161" style="font-weight: bold;text-decoration: underline;">HA601161</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: SiHa cell lysate<br />Lane 3: HeLa cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 65 kDa<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601161" style="font-weight: bold;text-decoration: underline;">HA601161</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SLC39A8 on different lysates with Mouse anti-SLC39A8 antibody (HA601161) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: SiHa cell lysate
    Lane 3: HeLa cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 50 kDa
    Observed band size: 65 kDa

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601161) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of SLC39A8 on different lysates with Mouse anti-SLC39A8 antibody (<a href="/products/HA601161" style="font-weight: bold;text-decoration: underline;">HA601161</a>) at 1/1,000 dilution.<br /><br />Lane 1: HUVEC cell lysate (30 µg/Lane)<br />Lane 2: NIH/3T3 cell lysate (30 µg/Lane)<br />Lane 3: RAW264.7 cell lysate (20 µg/Lane)<br />Lane 4: PC-12 cell lysate (26 µg/Lane)<br /><br />Predicted band size: 50 kDa<br />Observed band size: 65/130 kDa<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601161" style="font-weight: bold;text-decoration: underline;">HA601161</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SLC39A8 on different lysates with Mouse anti-SLC39A8 antibody (HA601161) at 1/1,000 dilution.

    Lane 1: HUVEC cell lysate (30 µg/Lane)
    Lane 2: NIH/3T3 cell lysate (30 µg/Lane)
    Lane 3: RAW264.7 cell lysate (20 µg/Lane)
    Lane 4: PC-12 cell lysate (26 µg/Lane)

    Predicted band size: 50 kDa
    Observed band size: 65/130 kDa

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601161) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"