Skip to content

SLC40A1 Mouse Monoclonal Antibody [A10A1]

Usd: 350

Specification

Bulk Order Quote | Customization Request

Catalog# HA601178

SLC40A1 Mouse Monoclonal Antibody [A10A1]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SLC40A1 Mouse Monoclonal Antibody [A10A1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within human SLC40A1 aa 257-306 / 571.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 63 kDa

Positive Control

HEK-293 cell lysate, HepG2 cell lysate, A375 cell lysate, NCCIT cell lysate, Caco-2 cell lysate, SW480 cell lysate.

Conjugation

unconjugated

Clone Number

A10A1

RRID

AB_3071897

Reactivity Data

WB
Human
Tested Tested
Mouse
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Ferroportin-1, also known as solute carrier family 40 member 1 (SLC40A1) or iron-regulated transporter 1 (IREG1), is a protein that in humans is encoded by the SLC40A1 gene, and is part of the Ferroportin (Fpn) Family. Ferroportin is a transmembrane protein that transports iron from the inside of a cell to the outside of the cell. Ferroportin is the only known iron exporter. After dietary iron is absorbed into the cells of the small intestine, ferroportin allows that iron to be transported out of those cells and into the bloodstream. Fpn also mediates the efflux of iron recycled from macrophages resident in the spleen and liver. Ferroportin is regulated by hepcidin, a hormone produced by the liver; hepcidin binds to Fpn and limits its iron-efflux activity, thereby reducing iron delivery to the blood plasma. Therefore, the interaction between Fpn and hepcidin controls systemic iron homeostasis.

Background References

1. Hao L et al. SLC40A1 Mediates Ferroptosis and Cognitive Dysfunction in Type 1 Diabetes. Neuroscience. 2021 May

2. Huang B et al. Dapagliflozin Ameliorates Renal Tubular Ferroptosis in Diabetes via SLC40A1 Stabilization. Oxid Med Cell Longev. 2022 Aug

Subcellular Location

Cell membrane, Basolateral cell membrane.

UNIPROT

SwissProt: Q9NP59 Human

Synonyms

Ferroportin 1 antibody

Ferroportin antibody

Ferroportin-1 antibody

FPN1 antibody

HFE4 antibody

IREG1 antibody

iron regulated gene 1 antibody

Iron regulated transporter 1 antibody

Iron-regulated transporter 1 antibody

MST079 antibody

Expand

Images

  • Western blot analysis of SLC40A1 on different lysates with Mouse anti-SLC40A1 antibody (<a href="/products/HA601178" style="font-weight: bold;text-decoration: underline;">HA601178</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: A375 cell lysate<br />Lane 4: NCCIT cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 63 kDa<br /><br />Exposure time: 1 minute 2 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601178" style="font-weight: bold;text-decoration: underline;">HA601178</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG for IP Nano-Secondary Antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SLC40A1 on different lysates with Mouse anti-SLC40A1 antibody (HA601178) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: A375 cell lysate
    Lane 4: NCCIT cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

    Exposure time: 1 minute 2 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601178) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of SLC40A1 on different lysates with Mouse anti-SLC40A1 antibody (<a href="/products/HA601178" style="font-weight: bold;text-decoration: underline;">HA601178</a>) at 1/1,000 dilution.<br /><br />Lane 1: Caco-2 cell lysate<br />Lane 2: SW480 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 63 kDa<br />Observed band size: 63 kDa<br /><br />Exposure time: 3 minutes 10 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601178" style="font-weight: bold;text-decoration: underline;">HA601178</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG for IP Nano-Secondary Antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SLC40A1 on different lysates with Mouse anti-SLC40A1 antibody (HA601178) at 1/1,000 dilution.

    Lane 1: Caco-2 cell lysate
    Lane 2: SW480 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

    Exposure time: 3 minutes 10 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601178) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

SLC40A1 Rabbit Polyclonal Antibody

Application: WB

Reactivity: Human

Conjugate: unconjugated