Human IL-21 Recombinant Mouse Monoclonal Antibody [PSH02-48] - BSA and Azide free (Capture)
Overview
Product Name
Human IL-21 Recombinant Mouse Monoclonal Antibody [PSH02-48] - BSA and Azide free (Capture)
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human IL-21 aa 25-162 (Q9HBE4).
Species Reactivity
Human
Validated Applications
ELISA(Cap)
Target Molecular Weight
Predicted band size: 18.6 kDa
Positive Control
Recombinant Human IL-21 Protein (HA211070).
Conjugation
unconjugated
Clone Number
PSH02-48
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [PSH02-49] to Human IL-21 (Capture) (HA601196).
Target
Function
IL-21 (Interleukin-21) is member of the family of cytokines that utilize the common gamma chain as a receptor subunit. This subunit is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. It exerts its biological effects through a heterodimeric receptor complex of the common gamma chain and the IL-21-specific IL-21 R. IL-21 supports Tfh cell development, immunoglobulin affinity maturation, plasma cell differentiation, B cell memory responses, and the migration of dendritic cells to draining lymph nodes. It costimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation.
Background References
1. Parrish-Novak J., Dillon S.R., Nelson A., Hammond A., Sprecher C., Gross J.A., Johnston J., Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature 408:57-63 (2000)
2. Strengell M., Julkunen I., Matikainen S. IFN-alpha regulates IL-21 and IL-21R expression in human NK and T cells. J. Leukoc. Biol. 76:416-422 (2004)
Subcellular Location
Secreted.
UNIPROT
Synonyms
CVID11 antibody
IL 21 antibody
IL-21 antibody
Il21 antibody
IL21_HUMAN antibody
Interleukin 21 antibody
Interleukin-21 antibody
interleukin-21 isoform antibody
Interleukin21 antibody
OTTHUMP00000164088 antibody
ExpandImages
-
![Sandwich ELISA analysis of Human IL-21 matched pair antibodies<br /><br />Capture: <a href="/products/HA601195" style="font-weight: bold;text-decoration: underline;">HA601195</a>, Human IL-21 Mouse mAb [PSH02-48]<br />Detector: <a href="/products/HA601196" style="font-weight: bold;text-decoration: underline;">HA601196</a>, Human IL-21 Mouse mAb [PSH02-49]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA601195" style="font-weight: bold;text-decoration: underline;">HA601195</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-21 protein (<a href="/products/HA211070" style="font-weight: bold;text-decoration: underline;">HA211070</a>) starting from 60,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA601196" style="font-weight: bold;text-decoration: underline;">HA601196</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA601195_1.jpg?v=20260908003422)
Sandwich ELISA analysis of Human IL-21 matched pair antibodies
Capture: HA601195, Human IL-21 Mouse mAb [PSH02-48]
Detector: HA601196, Human IL-21 Mouse mAb [PSH02-49]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA601195) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-21 protein (HA211070) starting from 60,000 pg/ml to 0 pg/ml and detect antibody (HA601196, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Biotin Conjugated Human IL-21 Recombinant Mouse Monoclonal Antibody [PSH02-49] - Detector
Application: ELISA(Det)
Reactivity: Human
Conjugate: Biotin
