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Oct4 Recombinant Mouse Monoclonal Antibody [A3-C11-R]

Usd: 350

Specification

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Catalog# HA601207

Oct4 Recombinant Mouse Monoclonal Antibody [A3-C11-R]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Oct4 Recombinant Mouse Monoclonal Antibody [A3-C11-R]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within full length human Oct4 protein.

Species Reactivity

Human

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 39 kDa

Positive Control

NCCIT cell lysates, NCCIT.

Conjugation

unconjugated

Clone Number

A3-C11-R

RRID

AB_3071926

Reactivity Data

WB IF-Cell
Human
Tested Tested
Tested Tested
Mouse
Rat

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

Target

Function

Oct-4 (POU5F1) is transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). It forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Oct-4 is critical for early embryogenesis and for embryonic stem cell pluripotency. It is expressed in developing brain and highest levels also found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.

Background References

1. Oct4 pseudogenes are transcribed in cancers."Suo G., Han J., Wang X., Zhang J., Zhao Y., Zhao Y., Dai J.Biochem. Biophys. Res. Commun. 337:1047-1051(2005)

2. Sumoylation of Oct4 enhances its stability, DNA binding, and transactivation."Wei F., Schoeler H.R., Atchison M.L.J. Biol. Chem. 282:21551-21560(2007)

3. Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1."Zhang Z., Liao B., Xu M., Jin Y.FASEB J. 21:3042-3051(2007)

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q01860 Human

Synonyms

Octamer binding transcription factor 4 antibody

MGC22487 antibody

Oct 3 antibody

Oct 4 antibody

Oct-3 antibody

Oct-4 antibody

OCT3 antibody

Oct4 antibody

Octamer binding protein 3 antibody

Octamer binding protein 4 antibody

Expand

Images

  • Western blot analysis of Oct4 on NCCIT cell lysates with Mouse anti-Oct4 antibody (<a href="/products/HA601207" style="font-weight: bold;text-decoration: underline;">HA601207</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 39 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 1 minute 59 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601207" style="font-weight: bold;text-decoration: underline;">HA601207</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Oct4 on NCCIT cell lysates with Mouse anti-Oct4 antibody (HA601207) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 39 kDa
    Observed band size: 45 kDa

    Exposure time: 1 minute 59 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601207) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Oct4 on different lysates with Mouse anti-Oct4 antibody (<a href="/products/HA601207" style="font-weight: bold;text-decoration: underline;">HA601207</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Oct4 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 39 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 18 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601207" style="font-weight: bold;text-decoration: underline;">HA601207</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Oct4 on different lysates with Mouse anti-Oct4 antibody (HA601207) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Oct4 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 39 kDa
    Observed band size: 42 kDa

    Exposure time: 18 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601207) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of NCCIT cells labeling Oct4 with Mouse anti-Oct4 antibody (<a href="/products/HA601207" style="font-weight: bold;text-decoration: underline;">HA601207</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Oct4 antibody (<a href="/products/HA601207" style="font-weight: bold;text-decoration: underline;">HA601207</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of NCCIT cells labeling Oct4 with Mouse anti-Oct4 antibody (HA601207) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Oct4 antibody (HA601207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"