APE1 Recombinant Mouse Monoclonal Antibody [12H2-R] (HA601212)
Catalog# HA601212
Mouse Monoclonal to APE1
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: LNCaP cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: HL-60 cell lysate (20 µg/Lane)
Lane 5: A431 cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Lane 7: C6 cell lysate (20 µg/Lane)
Lane 8: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601212) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Mouse monoclonal primary
Product Name
APE1 Recombinant Mouse Monoclonal Antibody [12H2-R] (HA601212)
Immunogen
Recombinant protein within human ape1 aa 20-318/318.
Host
Mouse
Positive Control
HeLa cell lysate, LNCaP cell lysate, HEK-293 cell lysate, HL-60 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, rat testis tissue lysate, A549, HeLa, human colon carcinoma tissue, human kidney tissue, mouse liver tissue, rat colon tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
12H2-R
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
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IHC-P
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1:200-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 36 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
APE1
SYNONYMS
AP endonuclease 1 antibody;AP endonuclease class I antibody;AP lyase antibody;APE 1 antibody;APE antibody;APE-1 antibody;APEN antibody;APEX 1 antibody;APEX antibody;APEX nuclease (multifunctional DNA repair enzyme) 1 antibody;Apex nuclease 1 antibody;APEX nuclease antibody;APEX1 antibody;APEX1_HUMAN antibody;Apurinic endonuclease antibody;Apurinic-apyrimidinic endonuclease 1 antibody;Apurinic/apyrimidinic (abasic) endonuclease antibody;Apurinic/apyrimidinic endonuclease 1 antibody;Apurinic/apyrimidinic exonuclease antibody;APX antibody;BAP1 antibody;Deoxyribonuclease (apurinic or apyrimidinic) antibody;DNA (apurinic or apyrimidinic site) lyase antibody;DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody;EC 4.2.99.18 antibody;HAP 1 antibody;HAP1 antibody;Human Apurinic endonuclease 1 antibody;MGC139790 antibody;Multifunctional DNA repair enzyme antibody;Redox factor 1 antibody;Redox factor-1 antibody;REF 1 antibody;REF 1 protein antibody;REF-1 antibody;REF1 antibody;REF1 protein antibody
SUBCELLULAR LOCATION
Nucleus. Endoplasmic reticulum, Cytoplasm.
FUNCTION
The role of transcription factors in the regulation of gene expression is well established. Although the activity of these factors can be regulated by phosphorylation, evidence has indicated regulation of DNA binding mediated by changes in reduction-oxidation (redox) status. Mutational analysis has identified a single conserved cysteine residue mapping within the DNA binding domains of Fos and Jun. Chemical oxidation or modification of this cysteine residue inhibits the DNA binding activity of Fos and Jun. A similar mode of regulation has been recently proposed for other nuclear transcription factors. Oxidation is reversible by these compounds or by a cellular redox/DNA repair protein identified originally as Ref-1 (redox factor 1). Ref-1 is identical to a previously characterized DNA repair enzyme designated HAP1, APE or APEX.
CITATIONS
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Aristolochic acid I aggravates oxidative stress-mediated apoptosis by inhibiting APE1/Nrf2/HO-1 signaling
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WB
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Human，Mouse
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Citation
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