Mouse anti-Human IgG Recombinant Mouse Monoclonal Antibody [A5D12-R] (HA601220)
Catalog# HA601220
Mouse Monoclonal to Mouse anti-Human IgG
Tested for
-
WB
-
ELISA
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Mouse anti-Human IgG on different lysates with Mouse anti-Human IgG antibody (HA601220) at 1/1,000 dilution.
Lane 1: Recombinant human IgG protein
Lane 2: Recombinant human IgM protein
Lysates/proteins at 50 ng/Lane.
Observed band size: 50 kDa
Exposure time: 2 minutes 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601220) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Recombinant human IgG protein
Lane 2: Recombinant human IgM protein
Lysates/proteins at 50 ng/Lane.
Observed band size: 50 kDa
Exposure time: 2 minutes 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601220) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
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