MGST1 Recombinant Mouse Monoclonal Antibody [14H2-R]
Catalog# HA601255
MGST1 Recombinant Mouse Monoclonal Antibody [14H2-R]
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WB
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IHC-P
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Human
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Rat
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unconjugated
Overview
Product Name
MGST1 Recombinant Mouse Monoclonal Antibody [14H2-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human MGST1 aa 1-144 / 155.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 18 kDa
Positive Control
SiHa cell lysate, HepG2 cell lysate, A549 cell lysate, U-937 cell lysate, MCF7 cell lysate, Rat liver tissue lysate, human liver tissue.
Conjugation
unconjugated
Clone Number
14H2-R
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | |
|---|---|---|---|
| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
DNA replication licensing factor MCM7 is a protein that in humans is encoded by the MCM7 gene.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Background References
1. The MGC Project Team. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14:2121-2127(2004).
Subcellular Location
Endoplasmic reticulum, Mitochondrion.
Synonyms
MGST1 antibody
Glutathione S transferase 12 antibody
GST12 antibody
MGST 1 antibody
MGST antibody
MGST I antibody
MGST1 antibody
MGST1_HUMAN antibody
Microsomal glutathione S-transferase 1 antibody
Microsomal GST 1 antibody
ExpandImages
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Western blot analysis of MGST1 on different lysates with Mouse anti-MGST1 antibody (HA601255) at 1/1,000 dilution.
Lane 1: SiHa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: U-937 cell lysate
Lane 5: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 15 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601255) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of MGST1 on different lysates with Mouse anti-MGST1 antibody (HA601255) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 18 kDa
Observed band size: 15 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601255) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-MGST1 antibody (HA601255) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601255) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"