p47-phox Recombinant Mouse Monoclonal Antibody [A6B10-R]
Catalog# HA601264
p47-phox Recombinant Mouse Monoclonal Antibody [A6B10-R]
-
WB
-
Human
-
unconjugated
Overview
Product Name
p47-phox Recombinant Mouse Monoclonal Antibody [A6B10-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human p47-phox aa 51-250.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
Ramos cell lysate, Raji cell lysate, Daudi cell lysate, HL-60 cell lysate.
Conjugation
unconjugated
Clone Number
A6B10-R
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
Target
Function
The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47phox and p67-phox. The cytosolic proteins p47phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47phox, suggesting that p47phox may faciliate the transport of p67-phox to the membrane.
Background References
1. Gong P. et. al. p47(phox) deficiency improves cognitive impairment and attenuates tau hyperphosphorylation in mouse models of AD. Alzheimers Res Ther. 2020 Nov
2. Kuhns DB. et. al. NCF1 (p47(phox))-deficient chronic granulomatous disease: comprehensive genetic and flow cytometric analysis. Blood Adv. 2019 Jan
Subcellular Location
Cytoplasm, cytosol, Membrane.
UNIPROT
Synonyms
47 kDa autosomal chronic granulomatous disease protein antibody
47 kDa neutrophil oxidase factor antibody
NADPH oxidase organizer 2 antibody
NCF 47K antibody
NCF-1 antibody
NCF-47K antibody
Ncf1 antibody
NCF1_HUMAN antibody
Neutrophil cytosol factor 1 antibody
Neutrophil cytosolic factor 1 antibody
ExpandImages
-
Western blot analysis of p47-phox on different lysates with Mouse anti-p47-phox antibody (HA601264) at 1/2,000 dilution.
Lane 1: Ramos cell lysate
Lane 2: Raji cell lysate
Lane 3: Daudi cell lysate
Lane 4: HL-60 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601264) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"