PAX8 Recombinant Mouse Monoclonal Antibody [6G5-R] (HA601270)
Catalog# HA601270
Mouse Monoclonal to PAX8
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Monkey
Lane 1: NIH:OVCAR-3 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lane 3: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601270) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Mouse monoclonal primary
Product Name
PAX8 Recombinant Mouse Monoclonal Antibody [6G5-R] (HA601270)
Immunogen
Synthetic peptide corresponding to human pax8 aa 400-450.
Host
Mouse
Positive Control
NIH:OVCAR-3 cell lysate, COS-1 cell lysate, human kidney tissue, human thyroid cancer tissue, human thyroid tissue, human renal clear cell carcinoma tissue, mouse thyroid tissue, NIH:OVCAR-3.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
6G5-R
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:500-1:2,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 48 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
PAX8
SYNONYMS
OTTHUMP00000158659 antibody;OTTHUMP00000158660 antibody;OTTHUMP00000203723 antibody;OTTHUMP00000203724 antibody;Paired box 8 antibody;Paired box gene 8 antibody;paired box homeotic gene 8 antibody;Paired box protein Pax 8 antibody;Paired box protein Pax-8 antibody;Paired domain gene 8 antibody;PAX 8 antibody;PAX8 antibody;PAX8_HUMAN antibody
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The PAX gene family has an important role in the formation of tissues and organs during embryonic development and maintaining the normal function of some cells after birth. The PAX genes give instructions for making proteins that attach themselves to certain areas of DNA. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. PAX8 releases the hormones important for regulating growth, brain development, and metabolism. Also functions in very early stages of kidney organogenesis, the müllerian system, and the thymus. Additionally, PAX8 is expressed in the renal excretory system, epithelial cells of the endocervix, endometrium, ovary, Fallopian tube, seminal vesicle, epididymis, pancreatic islet cells and lymphoid cells. PAX8 and other transcription factors play a role in binding to DNA and regulating the genes that drive thyroid hormone synthesis (Tg, TPO, Slc5a5 and Tshr). This PAX-8 antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalinfixed, paraffin-embedded tissue sections using IHC test methods.
CITATIONS
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Genome-wide alternation and effect of DNA methylation in the impairments of steroidogenesis and spermatogenesis after PM2. 5 exposure
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WB
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Mouse
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Citation
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