TROP2 Recombinant Mouse Monoclonal Antibody [A5E2-R]
Catalog# HA601282
TROP2 Recombinant Mouse Monoclonal Antibody [A5E2-R]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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HA610157
不含抗保成分
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unconjugated
Overview
Product Name
TROP2 Recombinant Mouse Monoclonal Antibody [A5E2-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human TROP2 aa 1-274 / 323 (Extracellular).
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 36 kDa
Positive Control
MCF7 cell lysate, A431 cell lysate, A431, human esophagus tissue.
Conjugation
unconjugated
Clone Number
A5E2-R
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
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IHC-P
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1:2,000
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FC
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1:1,000
Target
Function
TROP-2, also known as tumor-associated calcium signal transducer 2 (TACSTD2), pancreatic carcinoma marker protein GA733-1, membrane component chromosome 1, surface marker 1 (M1S1) or epithelial glycoprotein-1 (EGP-1), is a cell surface glycoprotein receptor. It is a single pass type I membrane protein containing one thryoglobulin type-1 domain, an epidermal growth factor-like repeat, a phosphatidylinositol binding site and tyrosine phosphorylation sites near the C-terminus. TROP-2 plays a role in tranducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multistratified epithelia. It is also highly expressed in several types of tumors and is involved in regulating the growth of carcinoma cells. Mutations in the gene encoding TROP-2 can result in gelatinous drop-like corneal dystrophy (GDLD) also referred to as lattice corneal dystrophy type III, an autosomal recessive disorder that causes severe visual impairment.
Background References
1. Lenárt S. et. al. Trop2: Jack of All Trades, Master of None. Cancers (Basel). 2020 Nov
2. Hsu EC. et. al. Trop2 is a driver of metastatic prostate cancer with neuroendocrine phenotype via PARP1. Proc Natl Acad Sci U S A. 2020 Jan
Subcellular Location
Membrane.
UNIPROT
Synonyms
Cell surface glycoprotein Trop 2 antibody
Cell surface glycoprotein Trop-2 antibody
Cell surface glycoprotein Trop2 antibody
Epithelial glycoprotein 1 antibody
GA733 1 antibody
GA7331 antibody
M1S 1 antibody
M1S1 antibody
Membrane component chromosome 1 surface marker 1 antibody
Pancreatic carcinoma marker protein GA733 1 antibody
ExpandImages
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Western blot analysis of TROP2 on different lysates with Mouse anti-TROP2 antibody (HA601282) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: A431 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 50 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601282) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A431 cells labeling TROP2 with Mouse anti-TROP2 antibody (HA601282) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-TROP2 antibody (HA601282) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Mouse anti-TROP2 antibody (HA601282) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601282) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of A431 cells labeling TROP2.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601282, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"