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Human IgG heavy chain Recombinant Mouse Monoclonal Antibody [54-76-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA601283

Human IgG heavy chain Recombinant Mouse Monoclonal Antibody [54-76-R] - BSA and Azide free

Application

  • WB

  • ELISA

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Human IgG heavy chain Recombinant Mouse Monoclonal Antibody [54-76-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human IgG heavy chain.

Species Reactivity

Human

Validated Applications

WB, ELISA, IHC-P

Positive Control

Recombinant Human IgG protein, human tonsil tissue.

Conjugation

unconjugated

Clone Number

54-76-R

RRID

AB_3096590

Reactivity Data

WB ELISA IHC-P
Human
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • ELISA

  • 1:1,000-1:20,000

  • IHC-P

  • 1:200

Target

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.

Background References

暂无

UNIPROT

SwissProt: P01857 Human

SwissProt: P01859 Human

SwissProt: P01860 Human

SwissProt: P01861 Human

Synonyms

FLJ39988 antibody

FLJ40036 antibody

FLJ40253 antibody

FLJ40587 antibody

FLJ40789 antibody

FLJ40834 antibody

G1m marker antibody

G2m marker antibody

G3m marker antibody

G4m marker antibody

Expand

Images

  • Western blot analysis of Human IgG heavy chain on Recombinant Human IgG protein with Mouse anti-Human IgG heavy chain antibody (<a href="/products/HA601283" style="font-weight: bold;text-decoration: underline;">HA601283</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 50 ng/Lane.<br /><br />Observed band size: 50 kDa<br /><br />Exposure time: 15 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601283" style="font-weight: bold;text-decoration: underline;">HA601283</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Human IgG heavy chain on Recombinant Human IgG protein with Mouse anti-Human IgG heavy chain antibody (HA601283) at 1/1,000 dilution.

    Lysates/proteins at 50 ng/Lane.

    Observed band size: 50 kDa

    Exposure time: 15 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Human IgG heavy chain antibody (<a href="/products/HA601283" style="font-weight: bold;text-decoration: underline;">HA601283</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601283" style="font-weight: bold;text-decoration: underline;">HA601283</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Human IgG heavy chain antibody (HA601283) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601283) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"