Human IgG heavy chain Recombinant Mouse Monoclonal Antibody [54-76-R]
Catalog# HA601283
Human IgG heavy chain Recombinant Mouse Monoclonal Antibody [54-76-R]
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WB
-
ELISA
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IHC-P
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Human
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unconjugated
Overview
Product Name
Human IgG heavy chain Recombinant Mouse Monoclonal Antibody [54-76-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human IgG heavy chain.
Species Reactivity
Human
Validated Applications
WB, ELISA, IHC-P
Positive Control
Recombinant Human IgG protein, human tonsil tissue.
Conjugation
unconjugated
Clone Number
54-76-R
RRID
Reactivity Data
| WB | ELISA | IHC-P | |
|---|---|---|---|
| Human |
|
|
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
-
ELISA
-
1:1,000-1:20,000
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IHC-P
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1:200
Target
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
Background References
暂无
UNIPROT
Synonyms
FLJ39988 antibody
FLJ40036 antibody
FLJ40253 antibody
FLJ40587 antibody
FLJ40789 antibody
FLJ40834 antibody
G1m marker antibody
G2m marker antibody
G3m marker antibody
G4m marker antibody
ExpandImages
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Western blot analysis of Human IgG heavy chain on Recombinant Human IgG protein with Mouse anti-Human IgG heavy chain antibody (HA601283) at 1/1,000 dilution.
Lysates/proteins at 50 ng/Lane.
Observed band size: 50 kDa
Exposure time: 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Human IgG heavy chain antibody (HA601283) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601283) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"