RBM15B Mouse Monoclonal Antibody [A10D12+A10D11]
Catalog# HA601308
RBM15B Mouse Monoclonal Antibody [A10D12+A10D11]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
RBM15B Mouse Monoclonal Antibody [A10D12+A10D11]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human RBM15B aa 1-200 / 890.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 97 kDa
Positive Control
HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
A10D12+A10D11
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex. Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist. Functions in the regulation of alternative or illicit splicing, possibly by regulating m6A methylation. Inhibits pre-mRNA splicing. Also functions as a mRNA export factor by acting as a cofactor for the nuclear export receptor NXF1.
Background References
1. Tan C et al. YY1-Targeted RBM15B Promotes Hepatocellular Carcinoma Cell Proliferation and Sorafenib Resistance by Promoting TRAM2 Expression in an m6A-Dependent Manner. Front Oncol. 2022 Apr
2. Wang T et al. N6-Methyladenosine regulator RBM15B acts as an independent prognostic biomarker and its clinical significance in uveal melanoma. Front Immunol. 2022 Aug
Subcellular Location
Nucleus, nucleoplasm, Nucleus speckle, Nucleus envelope.
Synonyms
1810017N16Rik antibody
Chromosome 3p21.1 gene sequence antibody
HUMAGCGB antibody
huOTT3 antibody
One twenty two protein 3 antibody
One-twenty two protein 3 antibody
OTT3 antibody
Putative RNA binding protein 15B antibody
Putative RNA-binding protein 15B antibody
RB15B_HUMAN antibody
ExpandImages
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Western blot analysis of RBM15B on different lysates with Mouse anti-RBM15B antibody (HA601308) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: Jurkat cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 100 kDa
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 27602518). The bands below 100 kDa may be caused by degradation.
Exposure time: 1 minute 21 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601308) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"