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EIF3H Recombinant Mouse Monoclonal Antibody [PSH04-49]

Usd: 350

Specification

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Catalog# HA601328

EIF3H Recombinant Mouse Monoclonal Antibody [PSH04-49]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

EIF3H Recombinant Mouse Monoclonal Antibody [PSH04-49]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human EIF3H aa 1-352 / 352.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 40 kDa

Positive Control

HEK-293 cell lysate, HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, HepG2 cell lysate, A549 cell lysate, SK-Br-3 cell lysate, MDA-MB-231 cell lysate, MDA-MB-468 cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human breast cancer tissue, human colon cancer tissue.

Conjugation

unconjugated

Clone Number

PSH04-49

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:200

Target

Function

Eukaryotic initiation factor 3 (eIF3) is a multiprotein complex that functions during the initiation phase of eukaryotic translation. It is essential for most forms of cap-dependent and cap-independent translation initiation. In humans, eIF3 consists of 13 nonidentical subunits (eIF3a-m) with a combined molecular weight of ~800 kDa, making it the largest translation initiation factor. The eIF3 complex is broadly conserved across eukaryotes, but the conservation of individual subunits varies across organisms. For instance, while most mammalian eIF3 complexes are composed of 13 subunits, budding yeast's eIF3 has only six subunits (eIF3a, b, c, g, i, j). Eukaryotic translation initiation factor 3 subunit H (eIF3h) is a protein that in humans is encoded by the EIF3H gene. eIF3h has been shown to interact with eIF3a.

Background References

1. Guo X et al. EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability. J Exp Clin Cancer Res. 2020 Aug

2. Zheng R et al. Exosomal circLPAR1 functions in colorectal cancer diagnosis and tumorigenesis through suppressing BRD4 via METTL3-eIF3h interaction. Mol Cancer. 2022 Feb

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: O15372 Human

SwissProt: Q91WK2 Mouse

SwissProt: Q6P9U8 Rat

Synonyms

eIF-3-gamma antibody

eIF3 gamma antibody

eIF3 p40 antibody

eIF3 p40 subunit antibody

eIF3h antibody

EIF3H_HUMAN antibody

EIF3S3 antibody

Eukaryotic translation initiation factor 3 subunit 3 antibody

Eukaryotic translation initiation factor 3 subunit H antibody

MGC102958 antibody

Images

  • Western blot analysis of EIF3H on different lysates with Mouse anti-EIF3H antibody (<a href="/products/HA601328" style="font-weight: bold;text-decoration: underline;">HA601328</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: HepG2 cell lysate<br />Lane 6: A549 cell lysate<br />Lane 7: SK-Br-3 cell lysate<br />Lane 8: MDA-MB-231 cell lysate<br />Lane 9: MDA-MB-468 cell lysate<br />Lane 10: MCF7 cell lysate<br />Lane 11: RAW264.7 cell lysate<br />Lane 12: NIH/3T3 cell lysate<br />Lane 13: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 40 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601328" style="font-weight: bold;text-decoration: underline;">HA601328</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of EIF3H on different lysates with Mouse anti-EIF3H antibody (HA601328) at 1/2,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: A549 cell lysate
    Lane 7: SK-Br-3 cell lysate
    Lane 8: MDA-MB-231 cell lysate
    Lane 9: MDA-MB-468 cell lysate
    Lane 10: MCF7 cell lysate
    Lane 11: RAW264.7 cell lysate
    Lane 12: NIH/3T3 cell lysate
    Lane 13: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601328) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EIF3H antibody (<a href="/products/HA601328" style="font-weight: bold;text-decoration: underline;">HA601328</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601328" style="font-weight: bold;text-decoration: underline;">HA601328</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EIF3H antibody (HA601328) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601328) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-EIF3H antibody (<a href="/products/HA601328" style="font-weight: bold;text-decoration: underline;">HA601328</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601328" style="font-weight: bold;text-decoration: underline;">HA601328</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-EIF3H antibody (HA601328) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601328) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"