EIF3H Recombinant Mouse Monoclonal Antibody [PSH04-49]
Catalog# HA601328
EIF3H Recombinant Mouse Monoclonal Antibody [PSH04-49]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
EIF3H Recombinant Mouse Monoclonal Antibody [PSH04-49]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human EIF3H aa 1-352 / 352.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
HEK-293 cell lysate, HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, HepG2 cell lysate, A549 cell lysate, SK-Br-3 cell lysate, MDA-MB-231 cell lysate, MDA-MB-468 cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human breast cancer tissue, human colon cancer tissue.
Conjugation
unconjugated
Clone Number
PSH04-49
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IHC-P
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1:200
Target
Function
Eukaryotic initiation factor 3 (eIF3) is a multiprotein complex that functions during the initiation phase of eukaryotic translation. It is essential for most forms of cap-dependent and cap-independent translation initiation. In humans, eIF3 consists of 13 nonidentical subunits (eIF3a-m) with a combined molecular weight of ~800 kDa, making it the largest translation initiation factor. The eIF3 complex is broadly conserved across eukaryotes, but the conservation of individual subunits varies across organisms. For instance, while most mammalian eIF3 complexes are composed of 13 subunits, budding yeast's eIF3 has only six subunits (eIF3a, b, c, g, i, j). Eukaryotic translation initiation factor 3 subunit H (eIF3h) is a protein that in humans is encoded by the EIF3H gene. eIF3h has been shown to interact with eIF3a.
Background References
1. Guo X et al. EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability. J Exp Clin Cancer Res. 2020 Aug
2. Zheng R et al. Exosomal circLPAR1 functions in colorectal cancer diagnosis and tumorigenesis through suppressing BRD4 via METTL3-eIF3h interaction. Mol Cancer. 2022 Feb
Subcellular Location
Cytoplasm.
Synonyms
eIF-3-gamma antibody
eIF3 gamma antibody
eIF3 p40 antibody
eIF3 p40 subunit antibody
eIF3h antibody
EIF3H_HUMAN antibody
EIF3S3 antibody
Eukaryotic translation initiation factor 3 subunit 3 antibody
Eukaryotic translation initiation factor 3 subunit H antibody
MGC102958 antibody
Images
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Western blot analysis of EIF3H on different lysates with Mouse anti-EIF3H antibody (HA601328) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HepG2 cell lysate
Lane 6: A549 cell lysate
Lane 7: SK-Br-3 cell lysate
Lane 8: MDA-MB-231 cell lysate
Lane 9: MDA-MB-468 cell lysate
Lane 10: MCF7 cell lysate
Lane 11: RAW264.7 cell lysate
Lane 12: NIH/3T3 cell lysate
Lane 13: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601328) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EIF3H antibody (HA601328) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601328) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-EIF3H antibody (HA601328) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601328) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"