LAIR1 Recombinant Mouse Monoclonal Antibody [PSH04-50]
Catalog# HA601329
LAIR1 Recombinant Mouse Monoclonal Antibody [PSH04-50]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
LAIR1 Recombinant Mouse Monoclonal Antibody [PSH04-50]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human LAIR1 aa 166-287 / 287.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 32 kDa
Positive Control
THP-1 cell lysate, HL-60 cell lysate, human lymph node tissue.
Conjugation
unconjugated
Clone Number
PSH04-50
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
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IHC-P
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1:200
Target
Function
Leukocyte-associated immunoglobulin-like receptor 1 is a protein that in humans is encoded by the LAIR1 gene.[5][6] LAIR1 has also been designated as CD305 (cluster of differentiation 305). The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including NK cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily.
Background References
1. Van Laethem F et al. LAIR1, an ITIM-Containing Receptor Involved in Immune Disorders and in Hematological Neoplasms. Int J Mol Sci. 2022 Dec
2. Peng DH et al. Collagen promotes anti-PD-1/PD-L1 resistance in cancer through LAIR1-dependent CD8+ T cell exhaustion. Nat Commun. 2020 Sep
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
5133400O11Rik antibody
CD305 antibody
D7Bwg0421e antibody
hLAIR1 antibody
LAIR 1 antibody
LAIR-1 antibody
LAIR1 antibody
LAIR1 protein antibody
LAIR1_HUMAN antibody
Leukocyte associated Ig like receptor 1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of LAIR1 on different lysates with Mouse anti-LAIR1 antibody (HA601329) at 1/2,000 dilution.
Lane 1: THP-1 cell lysate (20 µg/Lane)
Lane 2: HL-60 cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (negative) (20 µg/Lane)
Predicted band size: 32 kDa
Observed band size: 40-45 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601329) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-LAIR1 antibody (HA601329) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601329) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue (negative) with Mouse anti-LAIR1 antibody (HA601329) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601329) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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