Human SAA1 Mouse Monoclonal Antibody [A10E6] - BSA and Azide free (Detector)
Overview
Product Name
Human SAA1 Mouse Monoclonal Antibody [A10E6] - BSA and Azide free (Detector)
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human SAA1 aa 19-122 (P0DJI8).
Species Reactivity
Human
Validated Applications
ELISA(Det)
Molecular Weight
Predicted band size: 13.5 kDa
Positive Control
Recombinant human SAA protein (HA210927).
Conjugation
unconjugated
Clone Number
A10E6
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG1
Purification Method
Immunogen affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [A10E7] to Human SAA1 (Capture) (HA601333) and recombinant standard Human SAA1 (HA210927). The reference range value is 329-80,000 pg/mL.
Target
Function
Serum amyloid A1 (SAA1) is a protein that in humans is encoded by the SAA1 gene. SAA1 is a major acute-phase protein mainly produced by hepatocytes in response to infection, tissue injury and malignancy. When released into blood circulation, SAA1 is present as an apolipoprotein associated with high-density lipoprotein (HDL). SAA1 is a major precursor of amyloid A (AA), the deposit of which leads to inflammatory amyloidosis. As a result, SAA1 has been a clinical indicator and reliable biomarker for inflammatory diseases, chronic metabolic disorders and late-stage malignancy. Inflammatory amyloidosis results from chronic inflammation with increased production of SAA1, which is a major precursor of amyloid A fibril deposit in various tissues. SAA1 has been extensively studied for its binding to HDL, with results suggesting a role in lipid metabolism. During the acute-phase response, elevated levels of SAA1 in the plasma displaces ApoA-I and becomes a major apolipoprotein of HDL.
Background References
1. Takehara M et al. Cancer-associated adipocytes promote pancreatic cancer progression through SAA1 expression. Cancer Sci. 2020 Aug
2. Xiao Y et al. SAA1 deficiency alleviates cardiac remodeling by inhibiting NF-κB/p38/JNK and TGFβ/Smad pathways. FASEB J. 2023 May
Subcellular Location
Secreted.
UNIPROT
Synonyms
amyloid A, serum antibody
Amyloid fibril protein AA antibody
Amyloid protein A antibody
MGC111216 antibody
PIG4 antibody
SAA antibody
Serum amyloid A protein antibody
serum amyloid A-1 protein antibody
serum amyloid A1 antibody
TP53I4 antibody
ExpandImages
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Sandwich ELISA analysis of Human SAA matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA601333) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant human SAA protein (HA210927) starting from 80000 pg/ml to 0 pg/ml and detect antibody (HA601334, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
The concentrations of SAA were measured in duplicates, interpolated from the SAA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human SAA1 Mouse Monoclonal Antibody [A10E7] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
SAA1 Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human,Mouse
Conjugate: unconjugated
