Human CD3 Recombinant Mouse Monoclonal Antibody [PSH04-92]
Catalog# HA601337
Human CD3 Recombinant Mouse Monoclonal Antibody [PSH04-92]
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FC
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Human
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unconjugated
Overview
Product Name
Human CD3 Recombinant Mouse Monoclonal Antibody [PSH04-92]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human CD3 23-126.
Species Reactivity
Human
Validated Applications
FC
Molecular Weight
Predicted band size: 23 kDa
Positive Control
Human peripheral blood lymphocytes.
Conjugation
unconjugated
Clone Number
PSH04-92
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
FC
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1:1,000
Target
Function
CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). It is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. These chains associate with the T-cell receptor (TCR) and the CD3-zeta (ζ-chain) to generate an activation signal in T lymphocytes. The TCR, CD3-zeta, and the other CD3 molecules together constitute the TCR complex.
Background References
1. LeFevre JD et al. Anti-CD3 monoclonal antibodies for the prevention and treatment of type 1 diabetes: A literature review. Am J Health Syst Pharm. 2022 Nov
2. Chen Y et al. Cholesterol inhibits TCR signaling by directly restricting TCR-CD3 core tunnel motility. Mol Cell. 2022 Apr
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD3 epsilon antibody
CD3e antibody
CD3e antigen antibody
CD3e antigen epsilon polypeptide (TiT3 complex) antibody
CD3E antigen epsilon polypeptide antibody
CD3E antigen, epsilon subunit antibody
CD3e molecule epsilon antibody
CD3e molecule, epsilon (CD3 TCR complex) antibody
CD3e molecule, epsilon (CD3-TCR complex) antibody
CD3E_HUMAN antibody
ExpandImages
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Flow cytometric analysis of human peripheral blood lymphocytes labeling Human CD3.
Cells were stained with the primary antibody (HA601337, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Percp-Cy5.5 Conjugated Human CD3 Recombinant Mouse Monoclonal Antibody [PSH04-92]
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APC Conjugated Human CD3 Recombinant Mouse Monoclonal Antibody [PSH04-92]
Application: FC
Reactivity: Human
Conjugate: APC
PE Conjugated Human CD3 Recombinant Mouse Monoclonal Antibody [PSH04-92]
Application: FC
Reactivity: Human
Conjugate: PE
