Human CD8 alpha Recombinant Mouse Monoclonal Antibody [PSH04-94]
Catalog# HA601339
Human CD8 alpha Recombinant Mouse Monoclonal Antibody [PSH04-94]
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FC
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Human
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unconjugated
Overview
Product Name
Human CD8 alpha Recombinant Mouse Monoclonal Antibody [PSH04-94]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human CD8a aa 22-182.
Species Reactivity
Human
Validated Applications
FC
Molecular Weight
Predicted band size: 26 kDa
Positive Control
Human peripheral blood lymphocytes.
Conjugation
unconjugated
Clone Number
PSH04-94
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
FC
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1:1,000
Target
Function
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen-presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Two alternative transcripts encoding distinct isoforms, one membrane associated and one secreted, have been identified.
Background References
1. Zheng Z et al. CD8A as a Prognostic and Immunotherapy Predictive Biomarker Can Be Evaluated by MRI Radiomics Features in Bladder Cancer. Cancers (Basel). 2022 Oct
2. Tanemoto S et al. Single-cell transcriptomics of human gut T cells identifies cytotoxic CD4(+)CD8A(+) T cells related to mouse CD4 cytotoxic T cells. Front Immunol. 2022 Oct
Subcellular Location
Cell membrane; Secreted.
UNIPROT
Synonyms
alpha polypeptide (p32) antibody
CD_antigen=CD8a antibody
CD8 antibody
CD8 antigen alpha polypeptide antibody
CD8 antigen alpha polypeptide (p32) antibody
CD8 antigen, alpha polypeptide (p32) antibody
CD8a antibody
CD8A antigen antibody
CD8A molecule antibody
CD8A_HUMAN antibody
ExpandImages
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Flow cytometric analysis of human peripheral blood lymphocytes labeling Human CD8 alpha.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601339, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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