Human CD16 Recombinant Mouse Monoclonal Antibody [PSH04-95]
Catalog# HA601340
Human CD16 Recombinant Mouse Monoclonal Antibody [PSH04-95]
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FC
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Human
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unconjugated
Overview
Product Name
Human CD16 Recombinant Mouse Monoclonal Antibody [PSH04-95]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within
Species Reactivity
Human
Validated Applications
FC
Molecular Weight
Predicted band size: 29 kDa
Positive Control
Human peripheral blood granulocytes.
Conjugation
unconjugated
Clone Number
PSH04-95
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
FC
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1:1,000
Target
Function
CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction. The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16. CD16 is the type III Fcγ receptor. In humans, it exists in two different forms: FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which have 96% sequence similarity in the extracellular immunoglobulin binding regions. While FcγRIIIa is expressed on mast cells, macrophages, and natural killer cells as a transmembrane receptor, FcγRIIIb is only expressed on neutrophils. In addition, FcγRIIIb is the only Fc receptor anchored to the cell membrane by a glycosyl-phosphatidylinositol (GPI) linker, and also plays a significant role in triggering calcium mobilization and neutrophil degranulation. FcγRIIIa and FcγRIIIb together are able to activate degranulation, phagocytosis, and oxidative burst, which allows neutrophils to clear opsonized pathogens.
Background References
1. Liu X et al. CD16(+) fibroblasts foster a trastuzumab-refractory microenvironment that is reversed by VAV2 inhibition. Cancer Cell. 2022 Nov
2. Cocker ATH et al. The CD56-CD16+ NK cell subset in chronic infections. Biochem Soc Trans. 2023 Jun
Subcellular Location
Cell membrane, Secreted.
UNIPROT
Synonyms
CD 16 antibody
CD 16a antibody
CD16 antibody
CD16a antibody
CD16a antigen antibody
CD16B antibody
CD16b antigen antibody
Fc fragment of IgG antibody
Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
Fc fragment of IgG low affinity IIIa receptor antibody
ExpandImages
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Flow cytometric analysis of human peripheral blood granulocytes labeling Human CD16.
Cells were stained with the primary antibody (HA601340, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (black). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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