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Kindlin 2 Mouse Monoclonal Antibody [A10G12]

Usd: 350

Specification

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Catalog# HA601343

Kindlin 2 Mouse Monoclonal Antibody [A10G12]

Application

  • WB

  • IF-Cell

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Kindlin 2 Mouse Monoclonal Antibody [A10G12]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within human Kindlin 2 aa 301-350 / 680.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IP

Molecular Weight

Predicted band size: 78 kDa

Positive Control

MDA-MB-231 cell lysate, Saos-2 cell lysate, mouse heart tissue lysate, mouse lung tissue lysate, rat heart tissue lysate, rat lung tissue lysate, MDA-MB-231.

Conjugation

unconjugated

Clone Number

A10G12

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

  • IP

  • 1-2μg/sample

Target

Function

Fermitin family homolog 2 (FERMT2) also known as pleckstrin homology domain-containing family C member 1 (PLEKHC1) or kindlin-2 is a protein that in humans is encoded by the FERMT2 gene. Kindlin-2 is the first of the kindlin protein to be discovered in 1994. It was detected in a screen for epidermal growth factor (EGF)-induced mRNAs and initially named mitogen-inducible gene 2 (Mig-2) protein. FERMT2 is a component of extracellular matrix structures in mammalian cells and is required for proper control of cell shape change. A major task of kindlins is to regulate the activation of integrins. FERMT2 has been shown to interact with FBLIM1.

Background References

1. Gao H et al. Kindlin-2 haploinsufficiency protects against fatty liver by targeting Foxo1 in mice. Nat Commun. 2022 Feb

2. Wu X et al. Kindlin-2 preserves integrity of the articular cartilage to protect against osteoarthritis. Nat Aging. 2022 Apr

Subcellular Location

Cytoplasm, Nucleus, Cell surface.

UNIPROT

SwissProt: Q96AC1 Human

SwissProt: Q8CIB5 Mouse

Entrez Gene: 289992 Rat

Synonyms

FLJ34213 antibody

FLJ44462 antibody

UNC112 antibody

FERM2_HUMAN antibody

Fermitin family homolog 2 antibody

Fermt2 antibody

KIND2 antibody

Kindlin-2 antibody

MIG-2 antibody

MIG2 antibody

Expand

Images

  • Western blot analysis of Kindlin 2 on different lysates with Mouse anti-Kindlin 2 antibody (<a href="/products/HA601343" style="font-weight: bold;text-decoration: underline;">HA601343</a>) at 1/1,000 dilution.<br /><br />Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)<br />Lane 2: Saos-2 cell lysate (20 µg/Lane)<br />Lane 3: Mouse heart tissue lysate (40 µg/Lane)<br />Lane 4: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 5: Rat heart tissue lysate (40 µg/Lane)<br />Lane 6: Rat lung tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 78 kDa<br />Observed band size: 72 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601343" style="font-weight: bold;text-decoration: underline;">HA601343</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Kindlin 2 on different lysates with Mouse anti-Kindlin 2 antibody (HA601343) at 1/1,000 dilution.

    Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)
    Lane 2: Saos-2 cell lysate (20 µg/Lane)
    Lane 3: Mouse heart tissue lysate (40 µg/Lane)
    Lane 4: Mouse lung tissue lysate (40 µg/Lane)
    Lane 5: Rat heart tissue lysate (40 µg/Lane)
    Lane 6: Rat lung tissue lysate (40 µg/Lane)

    Predicted band size: 78 kDa
    Observed band size: 72 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601343) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of MDA-MB-231 cells labeling Kindlin 2 with Mouse anti-Kindlin 2 antibody (<a href="/products/HA601343" style="font-weight: bold;text-decoration: underline;">HA601343</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Kindlin 2 antibody (<a href="/products/HA601343" style="font-weight: bold;text-decoration: underline;">HA601343</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of MDA-MB-231 cells labeling Kindlin 2 with Mouse anti-Kindlin 2 antibody (HA601343) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Kindlin 2 antibody (HA601343) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

  • Kindlin 2 was immunoprecipitated from 0.2 mg MDA-MB-231 cell lysate with <a href="/products/HA601343" style="font-weight: bold;text-decoration: underline;">HA601343</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA601343" style="font-weight: bold;text-decoration: underline;">HA601343</a> at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: MDA-MB-231 cell lysate (input)<br />Lane 2: <a href="/products/HA601343" style="font-weight: bold;text-decoration: underline;">HA601343</a> IP in MDA-MB-231 cell lysate<br />Lane 3: Mouse IgG instead of <a href="/products/HA601343" style="font-weight: bold;text-decoration: underline;">HA601343</a> in MDA-MB-231 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 38 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    Kindlin 2 was immunoprecipitated from 0.2 mg MDA-MB-231 cell lysate with HA601343 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601343 at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: MDA-MB-231 cell lysate (input)
    Lane 2: HA601343 IP in MDA-MB-231 cell lysate
    Lane 3: Mouse IgG instead of HA601343 in MDA-MB-231 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 38 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation