CCK4 Mouse Monoclonal Antibody [A11A9]
Catalog# HA601344
CCK4 Mouse Monoclonal Antibody [A11A9]
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WB
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IF-Cell
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
CCK4 Mouse Monoclonal Antibody [A11A9]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human CCK4 aa 31-704 / 1,070.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 118 kDa
Positive Control
HeLa cell lysate, Caco-2 cell lysate, Jurkat cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, COS-1 cell lysate, HeLa, NIH/3T3, PC-12.
Conjugation
unconjugated
Clone Number
A11A9
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:500
Target
Function
Tyrosine-protein kinase-like 7 also known as colon carcinoma kinase 4 (CCK4) is a receptor tyrosine kinase that in humans is encoded by the PTK7 gene. Receptor protein tyrosine kinases transduce extracellular signals across the cell membrane. A subgroup of these kinases lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. The protein encoded by this gene an intracellular domain with tyrosine kinase homology and may function as a cell adhesion molecule. This gene is thought to be expressed in colon carcinomas but not in normal colon, and therefore may be a marker for or may be involved in tumor progression. Four transcript variants encoding four different isoforms have been found for this gene. PTK7 serves as a context-dependent signalling switch for the Wnt pathways (particularly in planar cell polarity related functions such as convergent extension and neural crest cell migration) and appears to have similar functions for plexin and Flt-1 pathways. PTK7 was identified to be highly expressed in colon cancer by Saha et al. using serial analysis of gene expression (LongSAGE).
Background References
1. Yun J et al. Senescent cells perturb intestinal stem cell differentiation through Ptk7 induced noncanonical Wnt and YAP signaling. Nat Commun. 2023 Jan
2. Kong C et al. MTX-13, a Novel PTK7-Directed Antibody-Drug Conjugate with Widened Therapeutic Index Shows Sustained Tumor Regressions for a Broader Spectrum of PTK7-Positive Tumors. Mol Cancer Ther. 2023 Oct
Subcellular Location
Membrane, Cell junction.
Synonyms
8430404F20Rik antibody
CCK 4 antibody
CCK-4 antibody
Colon carcinoma kinase 4 antibody
Human colon carcinoma kinase 4 (CCK4) mRNA complete cds antibody
Inactive tyrosine-protein kinase 7 antibody
mPTK7/CCK4 antibody
OTTHUMP00000217016 antibody
Protein tyrosine kinase PTK 7 antibody
Protein-tyrosine kinase 7 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CCK4 on different lysates with Mouse anti-CCK4 antibody (HA601344) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate (negative)
Lane 3: Caco-2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: C2C12 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: C6 cell lysate
Lane 9: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 118 kDa
Observed band size: 150 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601344) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling CCK4 with Mouse anti-CCK4 antibody (HA601344) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CCK4 antibody (HA601344) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of NIH/3T3 cells labeling CCK4 with Mouse anti-CCK4 antibody (HA601344) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CCK4 antibody (HA601344) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of PC-12 cells labeling CCK4 with Mouse anti-CCK4 antibody (HA601344) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CCK4 antibody (HA601344) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"