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Rbm15 Mouse Monoclonal Antibody [A10H4]

Usd: 350

Specification

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Catalog# HA601347

Rbm15 Mouse Monoclonal Antibody [A10H4]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

Conjugation

  • unconjugated

Overview

Product Name

Rbm15 Mouse Monoclonal Antibody [A10H4]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human RBM15 aa 450-850.

Product Specificity

RBM15 (A10H4) Mouse mAb recognizes endogenous levels of total RBM15 protein. It does not recognize RBM15B.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 107 kDa

Positive Control

HeLa cell lysate, 293T cell lysate, 22RV1 cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate, C2C12 cell lysate, RAW264.7 cell lysate, human breast cancer tissue, human liver cancer tissue, mouse lymph nodes tissue, rat lymph nodes tissue.

Conjugation

unconjugated

Clone Number

A10H4

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:100

Target

Function

RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as hematopoietic cell homeostasis, alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (By similarity). Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex. Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist. Required for the development of multiple tissues, such as the maintenance of the homeostasis of long-term hematopoietic stem cells and for megakaryocyte (MK) and B-cell differentiation (By similarity). Regulates megakaryocyte differentiation by regulating alternative splicing of genes important for megakaryocyte differentiation; probably regulates alternative splicing via m6A regulation.

Background References

1. Patil D.P., Chen C.K., Pickering B.F., Chow A., Jackson C., Guttman M., Jaffrey S.R. m(6)A RNA methylation promotes XIST-mediated transcriptional repression. Nature 537:369-373 (2016).

2. Zhang L., Tran N.T., Su H., Wang R., Lu Y., Tang H., Aoyagi S. Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing. Elife 4:0-0 (2015)

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q96T37 Human

SwissProt: Q6PHZ5 Mouse

SwissProt: M0R3Z8 Rat

SwissProt: A0A0D9S6H4 Green monkey

Synonyms

One twenty two protein antibody

One-twenty two protein 1 antibody

OTT antibody

OTT1 antibody

Putative RNA-binding protein 15 antibody

RBM15 antibody

RBM15_HUMAN antibody

RNA binding motif protein 15 antibody

RNA-binding motif protein 15 antibody

Images

  • Western blot analysis of Rbm15 on different lysates with Mouse anti-Rbm15 antibody (<a href="/products/HA601347" style="font-weight: bold;text-decoration: underline;">HA601347</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: 22RV1 cell lysate<br />Lane 4: C6 cell lysate<br />Lane 5: PC-12 cell lysate<br />Lane 6: COS-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 107 kDa<br />Observed band size: 107 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601347" style="font-weight: bold;text-decoration: underline;">HA601347</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Rbm15 on different lysates with Mouse anti-Rbm15 antibody (HA601347) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: 293T cell lysate
    Lane 3: 22RV1 cell lysate
    Lane 4: C6 cell lysate
    Lane 5: PC-12 cell lysate
    Lane 6: COS-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 107 kDa
    Observed band size: 107 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601347) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Rbm15 on different lysates with Mouse anti-Rbm15 antibody (<a href="/products/HA601347" style="font-weight: bold;text-decoration: underline;">HA601347</a>) at 1/1,000 dilution.<br /><br />Lane 1: C2C12 cell lysate<br />Lane 2: RAW264.7 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 107 kDa<br />Observed band size: 107 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601347" style="font-weight: bold;text-decoration: underline;">HA601347</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Rbm15 on different lysates with Mouse anti-Rbm15 antibody (HA601347) at 1/1,000 dilution.

    Lane 1: C2C12 cell lysate
    Lane 2: RAW264.7 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 107 kDa
    Observed band size: 107 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601347) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-Rbm15 antibody (<a href="/products/HA601347" style="font-weight: bold;text-decoration: underline;">HA601347</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601347" style="font-weight: bold;text-decoration: underline;">HA601347</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-Rbm15 antibody (HA601347) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601347) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-Rbm15 antibody (<a href="/products/HA601347" style="font-weight: bold;text-decoration: underline;">HA601347</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601347" style="font-weight: bold;text-decoration: underline;">HA601347</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-Rbm15 antibody (HA601347) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601347) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lymph nodes tissue with Mouse anti-Rbm15 antibody (<a href="/products/HA601347" style="font-weight: bold;text-decoration: underline;">HA601347</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601347" style="font-weight: bold;text-decoration: underline;">HA601347</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lymph nodes tissue with Mouse anti-Rbm15 antibody (HA601347) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601347) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat lymph nodes tissue with Mouse anti-Rbm15 antibody (<a href="/products/HA601347" style="font-weight: bold;text-decoration: underline;">HA601347</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601347" style="font-weight: bold;text-decoration: underline;">HA601347</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat lymph nodes tissue with Mouse anti-Rbm15 antibody (HA601347) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601347) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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