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COMP Mouse Monoclonal Antibody [A10H2]

Usd: 350

Specification

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Catalog# HA601354

COMP Mouse Monoclonal Antibody [A10H2]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

COMP Mouse Monoclonal Antibody [A10H2]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within human COMP aa 321-370.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 83 kDa

Positive Control

Mouse articular cartilage tissue lysate, Rat articular cartilage tissue lysate, HCT 116 cell lysate, mouse cartilage tissue, rat cartilage tissue.

Conjugation

unconjugated

Clone Number

A10H2

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:400

Target

Function

Cartilage oligomeric matrix protein (COMP), also known as thrombospondin-5, is an extracellular matrix (ECM) protein primarily present in cartilage. In humans it is encoded by the COMP gene. The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfide bonds. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). COMP is a marker of cartilage turnover. It is present in high quantities in fibrotic scars and systemic sclerosis, and it appears to have a role in vascular wall remodeling.

Background References

1. Cai X et al. COMP Improves Ang-II-Induced Atrial Fibrillation via TGF-beta Signaling Pathway. Cardiovasc Toxicol. 2023 Oct

2. Wang Y et al. COMP promotes pancreatic fibrosis by activating pancreatic stellate cells through CD36-ERK/AKT signaling pathways. Cell Signal. 2024 Jun

Subcellular Location

Secreted, extracellular space, extracellular matrix.

UNIPROT

SwissProt: P49747 Human

SwissProt: Q9R0G6 Mouse

SwissProt: P35444 Rat

Synonyms

cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple) antibody

Cartilage oligomeric matrix protein antibody

Cartilage oligomeric matrix protein precursor antibody

COMP antibody

COMP_HUMAN antibody

EDM 1 antibody

EDM1 antibody

EPD 1 antibody

EPD1 antibody

Epiphyseal dysplasia 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of COMP on different lysates with Mouse anti-COMP antibody (<a href="/products/HA601354" style="font-weight: bold;text-decoration: underline;">HA601354</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse articular cartilage tissue lysate (40 µg/Lane)<br />Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane)<br />Lane 3: Rat articular cartilage tissue lysate (40 µg/Lane)<br />Lane 4: Rat liver tissue lysate (negative) (40 µg/Lane)<br /><br />Predicted band size: 83 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601354" style="font-weight: bold;text-decoration: underline;">HA601354</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of COMP on different lysates with Mouse anti-COMP antibody (HA601354) at 1/1,000 dilution.

    Lane 1: Mouse articular cartilage tissue lysate (40 µg/Lane)
    Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane)
    Lane 3: Rat articular cartilage tissue lysate (40 µg/Lane)
    Lane 4: Rat liver tissue lysate (negative) (40 µg/Lane)

    Predicted band size: 83 kDa
    Observed band size: 100 kDa

    Exposure time: 3 minutes; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of COMP on different lysates with Mouse anti-COMP antibody (<a href="/products/HA601354" style="font-weight: bold;text-decoration: underline;">HA601354</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT 116 cell lysate (15 µg/Lane)<br />Lane 2: HCT 116 cell lysate (30 µg/Lane)<br /><br />Predicted band size: 83 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 3 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601354" style="font-weight: bold;text-decoration: underline;">HA601354</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of COMP on different lysates with Mouse anti-COMP antibody (HA601354) at 1/1,000 dilution.

    Lane 1: HCT 116 cell lysate (15 µg/Lane)
    Lane 2: HCT 116 cell lysate (30 µg/Lane)

    Predicted band size: 83 kDa
    Observed band size: 100 kDa

    Exposure time: 3 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse cartilage tissue with Mouse anti-COMP antibody (<a href="/products/HA601354" style="font-weight: bold;text-decoration: underline;">HA601354</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601354" style="font-weight: bold;text-decoration: underline;">HA601354</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cartilage tissue with Mouse anti-COMP antibody (HA601354) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Mouse anti-COMP antibody (<a href="/products/HA601354" style="font-weight: bold;text-decoration: underline;">HA601354</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601354" style="font-weight: bold;text-decoration: underline;">HA601354</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Mouse anti-COMP antibody (HA601354) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cartilage tissue with Mouse anti-COMP antibody (<a href="/products/HA601354" style="font-weight: bold;text-decoration: underline;">HA601354</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601354" style="font-weight: bold;text-decoration: underline;">HA601354</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cartilage tissue with Mouse anti-COMP antibody (HA601354) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Mouse anti-COMP antibody (<a href="/products/HA601354" style="font-weight: bold;text-decoration: underline;">HA601354</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601354" style="font-weight: bold;text-decoration: underline;">HA601354</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Mouse anti-COMP antibody (HA601354) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

COMP Rabbit Polyclonal Antibody

Application: WB

Reactivity: Human

Conjugate: unconjugated