Biotin Conjugated NuMA Mouse Monoclonal Antibody [PSH03-08] - Detector
Overview
Product Name
Biotin Conjugated NuMA Mouse Monoclonal Antibody [PSH03-08] - Detector
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human NuMA aa 1-200 / 2,115 (HA210958).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Molecular Weight
Predicted band size: 238 kDa kDa
Positive Control
Recombinant Human NuMA protein (HA210958).
Conjugation
Biotin
Clone Number
PSH03-08
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [PSH03-07] to Human NuMA (Capture) (HA601223) and Recombinant Human NuMA protein (HA210958) as the standard. The reference range value is 51-12,500 pg/ml.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division. Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles. Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner. In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle. During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation. Also required for proper orientation of the mitotic spindle during asymmetric cell divisions. Plays a role in mitotic MT aster assembly. Positively regulates TNKS protein localization to spindle poles in mitosis.
Background References
1. Zou YS. et. al. Novel t(5;11)(q32;q13.4) with NUMA1-PDGFRB fusion in a myeloid neoplasm with eosinophilia with response to imatinib mesylate. Cancer Genet. 2017 Apr
2. Torii T. et. al. NuMA1 promotes axon initial segment assembly through inhibition of endocytosis. J Cell Biol. 2020 Feb.
Subcellular Location
Nucleus, Cytoskeleton, Plasma membrane.
UNIPROT
Synonyms
Centrophilin stabilizes mitotic spindle in mitotic cells antibody
NMP 22 antibody
Nuclear matrix protein 22 antibody
Nuclear mitotic apparatus protein 1 antibody
Nuclear mitotic apparatus protein antibody
NUMA 1 antibody
NUMA antibody
NuMA protein antibody
NUMA1 antibody
NUMA1_HUMAN antibody
ExpandImages
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Sandwich ELISA analysis of human NuMA matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA601223) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human NuMA protein (HA210958) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA601224, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of native NuMA in Hela extract samples based on a 1000 µg/ml extract load.
Interpolated concentration of native NuMA was measured in duplicate at different sample concentrations and interpolated from the NuMA standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NuMA concentration was determined to be 5,378 pg/mL in Hela cell extract. -
Interpolated concentrations of spiked NuMA in cell culture media samples.
The concentrations of NuMA were measured in duplicates, interpolated from the NuMA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"