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TRPV1 Recombinant Mouse Monoclonal Antibody [PSH13-22]

Usd: 350

Specification

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Catalog# HA601435

TRPV1 Recombinant Mouse Monoclonal Antibody [PSH13-22]

Application

  • IHC-P

Reactivity

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

TRPV1 Recombinant Mouse Monoclonal Antibody [PSH13-22]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Rat TRPV1 aa 1-200.

Species Reactivity

Rat

Validated Applications

IHC-P

Molecular Weight

Predicted band size: 95 kDa

Positive Control

Rat dorsal root ganglia tissue.

Conjugation

unconjugated

Clone Number

PSH13-22

Reactivity Data

IHC-P
Rat
Tested Tested
Human
Not Recommended
Mouse
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:200

Target

Function

The transient receptor potential cation channel subfamily V member 1 (TRPV1), also known as the capsaicin receptor and the vanilloid receptor 1, is a protein that, in humans, is encoded by the TRPV1 gene. It was the first isolated member of the transient receptor potential vanilloid receptor proteins that in turn are a sub-family of the transient receptor potential protein group. This protein is a member of the TRPV group of transient receptor potential family of ion channels. Fatty acid metabolites with affinity for this receptor are produced by cyanobacteria, which diverged from eukaryotes at least 2000 million years ago (MYA). The function of TRPV1 is detection and regulation of body temperature. In addition, TRPV1 provides a sensation of scalding heat and pain (nociception). In primary afferent sensory neurons, it cooperates with TRPA1 (a chemical irritant receptor) to mediate the detection of noxious environmental stimuli.

Background References

1. Iftinca M et al. TRPV1-Targeted Drugs in Development for Human Pain Conditions. Drugs. 2021 Jan

2. Munjuluri S et al. Capsaicin and TRPV1 Channels in the Cardiovascular System: The Role of Inflammation. Cells. 2021 Dec

Subcellular Location

Postsynaptic cell membrane, Cell projection, dendritic spine membrane, Cell membrane.

UNIPROT

SwissProt: O35433 Rat

Synonyms

Capsaicin receptor antibody

DKFZp434K0220 antibody

osm 9 like TRP channel 1 antibody

Osm-9-like TRP channel 1 antibody

OTRPC1 antibody

Transient receptor potential cation channel subfamily V member 1 antibody

TRPV 1 antibody

Trpv1 antibody

TRPV1_HUMAN antibody

Vanilloid receptor 1 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglia tissue with Mouse anti-TRPV1 antibody (<a href="/products/HA601435" style="font-weight: bold;text-decoration: underline;">HA601435</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601435" style="font-weight: bold;text-decoration: underline;">HA601435</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglia tissue with Mouse anti-TRPV1 antibody (HA601435) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601435) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat skin tissue (negative) with Mouse anti-TRPV1 antibody (<a href="/products/HA601435" style="font-weight: bold;text-decoration: underline;">HA601435</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601435" style="font-weight: bold;text-decoration: underline;">HA601435</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue (negative) with Mouse anti-TRPV1 antibody (HA601435) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601435) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"