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CD45RO Recombinant Mouse Monoclonal Antibody [PSH13-94]

Usd: 350

Specification

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Catalog# HA601441

CD45RO Recombinant Mouse Monoclonal Antibody [PSH13-94]

Application

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CD45RO Recombinant Mouse Monoclonal Antibody [PSH13-94]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Species Reactivity

Human

Validated Applications

IHC-P, FC

Target Molecular Weight

Predicted band size: 131 kDa

Positive Control

Human colon cancer tissue, human liver tissue, human spleen tissue, human peripheral blood cells.

Conjugation

unconjugated

Clone Number

PSH13-94

Reactivity Data

IHC-P FC
Human
Tested Tested
Tested Tested
Mouse
Rat

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:10,000

  • FC

  • 1:1,000

Target

Function

Protein tyrosine phosphatase, receptor type, C also known as PTPRC is an enzyme that, in humans, is encoded by the PTPRC gene. PTPRC is also known as CD45 antigen (CD stands for cluster of differentiation), which was originally called leukocyte common antigen (LCA). PTPRC is a critical enzyme involved in regulating immune cell function. PTPRC is a transmembrane protein tyrosine phosphatase expressed on the surface of all nucleated hematopoietic cells, particularly lymphocytes. It plays a key role in the activation and differentiation of T cells, B cells, and other immune cells by modulating signaling pathways. It functions by dephosphorylating specific tyrosine residues on target proteins, thereby controlling various signaling processes essential for immune response and homeostasis. Naive T lymphocytes are typically positive for CD45RA, which includes only the A protein region. Activated and memory T lymphocytes express CD45RO, the shortest CD45 isoform, which lacks all three of the A, B, and C regions. This shortest isoform facilitates T cell activation.

Background References

1. Mohammadi Y et al. CD45RO+TILs: cellular biomarkers for larynx squamous cell carcinoma outcome. Braz J Otorhinolaryngol. 2022 Nov-Dec

2. Kandel A et al. Differential Expression of CD45RO and CD45RA in Bovine T Cells. Cells. 2022 Jun

Subcellular Location

Cell membrane, Membrane raft, Synapse.

UNIPROT

SwissProt: P08575-4 Human

Synonyms

CD45RO

CD45R0

Images

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-CD45RO antibody (<a href="/products/HA601441" style="font-weight: bold;text-decoration: underline;">HA601441</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601441" style="font-weight: bold;text-decoration: underline;">HA601441</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-CD45RO antibody (HA601441) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601441) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD45RO antibody (<a href="/products/HA601441" style="font-weight: bold;text-decoration: underline;">HA601441</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601441" style="font-weight: bold;text-decoration: underline;">HA601441</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD45RO antibody (HA601441) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601441) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD45RO antibody (<a href="/products/HA601441" style="font-weight: bold;text-decoration: underline;">HA601441</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601441" style="font-weight: bold;text-decoration: underline;">HA601441</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD45RO antibody (HA601441) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601441) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of human peripheral blood cells labeling CD45RO.<br /><br />Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (<a href="/products/HA601441" style="font-weight: bold;text-decoration: underline;">HA601441</a>, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 647 conjugate-Goat anti-Mouse IgG Secondary antibody (<a href="/products/HA1127" style="font-weight: bold;text-decoration: underline;">HA1127</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of human peripheral blood cells labeling CD45RO.

    Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601441, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 647 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1127) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"