CD45RO Recombinant Mouse Monoclonal Antibody [PSH13-94]
Catalog# HA601441
CD45RO Recombinant Mouse Monoclonal Antibody [PSH13-94]
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IHC-P
-
FC
-
Human
-
unconjugated
Overview
Product Name
CD45RO Recombinant Mouse Monoclonal Antibody [PSH13-94]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Species Reactivity
Human
Validated Applications
IHC-P, FC
Molecular Weight
Predicted band size: 131 kDa
Positive Control
Human colon cancer tissue, human liver tissue, human spleen tissue, human peripheral blood cells.
Conjugation
unconjugated
Clone Number
PSH13-94
Reactivity Data
| IHC-P | FC | |
|---|---|---|
| Human |
|
|
| Mouse | ||
| Rat |
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-P
-
1:10,000
-
FC
-
1:1,000
Target
Function
Protein tyrosine phosphatase, receptor type, C also known as PTPRC is an enzyme that, in humans, is encoded by the PTPRC gene. PTPRC is also known as CD45 antigen (CD stands for cluster of differentiation), which was originally called leukocyte common antigen (LCA). PTPRC is a critical enzyme involved in regulating immune cell function. PTPRC is a transmembrane protein tyrosine phosphatase expressed on the surface of all nucleated hematopoietic cells, particularly lymphocytes. It plays a key role in the activation and differentiation of T cells, B cells, and other immune cells by modulating signaling pathways. It functions by dephosphorylating specific tyrosine residues on target proteins, thereby controlling various signaling processes essential for immune response and homeostasis. Naive T lymphocytes are typically positive for CD45RA, which includes only the A protein region. Activated and memory T lymphocytes express CD45RO, the shortest CD45 isoform, which lacks all three of the A, B, and C regions. This shortest isoform facilitates T cell activation.
Background References
1. Mohammadi Y et al. CD45RO+TILs: cellular biomarkers for larynx squamous cell carcinoma outcome. Braz J Otorhinolaryngol. 2022 Nov-Dec
2. Kandel A et al. Differential Expression of CD45RO and CD45RA in Bovine T Cells. Cells. 2022 Jun
Subcellular Location
Cell membrane, Membrane raft, Synapse.
UNIPROT
Synonyms
CD45RO
CD45R0
Images
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-CD45RO antibody (HA601441) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601441) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD45RO antibody (HA601441) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601441) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD45RO antibody (HA601441) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601441) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of human peripheral blood cells labeling CD45RO.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601441, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 647 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1127) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"