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NF-M Recombinant Antibody [JM11-20] - Guinea pig IgG2 (Chimeric)

Usd: 350

Specification

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Catalog# HA601444

NF-M Recombinant Antibody [JM11-20] - Guinea pig IgG2 (Chimeric)

Application

  • IHC-Fr

  • WB

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

NF-M Recombinant Antibody [JM11-20] - Guinea pig IgG2 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human NEFM aa 651-692 / 916.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, WB

Molecular Weight

Predicted band size: 102 kDa

Positive Control

Mouse cerebellum tissue, Rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

JM11-20

Reactivity Data

IHC-Fr WB
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • WB

  • 1:5,000

Target

Function

Neurofilament-M (NF-M), for neurofilament medium polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease, and Alzheimer's disease.

Background References

1. Li D et al. NEFM DNA methylation correlates with immune infiltration and survival in breast cancer. Clin Epigenetics. 2021 May

2. Sohrabi N et al. Regulatory Role of Insulin on Endogenous L1 ORF1 and NEFM Gene Expression through PI3K Signaling Pathway Specifically in Neuroblastoma Cell Line. Iran J Public Health. 2023 Mar

Subcellular Location

Cell projection, Cytoplasm, Cytoskeleton, Intermediate filament.

UNIPROT

SwissProt: P08553 Mouse

SwissProt: P12839 Rat

Synonyms

150kDa medium antibody

NEF3 antibody

NEFM antibody

Neurofilament 3 antibody

NF160 antibody

NFM antibody

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (NF-M, <a href="/products/HA601444" style="font-weight: bold;text-decoration: underline;">HA601444</a>, Guinea pig, red); 1/500 (QKI-6, <a href="/products/HA723588" style="font-weight: bold;text-decoration: underline;">HA723588</a>, Rabbit, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500 (NF-M, HA601444, Guinea pig, red); 1/500 (QKI-6, HA723588, Rabbit, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (NF-M, <a href="/products/HA601444" style="font-weight: bold;text-decoration: underline;">HA601444</a>, Guinea pig, red); 1/500 (QKI-6, <a href="/products/HA723588" style="font-weight: bold;text-decoration: underline;">HA723588</a>, Rabbit, green)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500 (NF-M, HA601444, Guinea pig, red); 1/500 (QKI-6, HA723588, Rabbit, green)

    Antigen retrieval: Not required

  • Western blot analysis of NF-M on different lysates with Guinea pig anti-NF-M antibody (<a href="/products/HA601444" style="font-weight: bold;text-decoration: underline;">HA601444</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse liver tissue lysate (negative)<br />Lane 3: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 102 kDa<br />Observed band size: 140 kDa<br /><br />Exposure time: 8 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601444" style="font-weight: bold;text-decoration: underline;">HA601444</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (<a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NF-M on different lysates with Guinea pig anti-NF-M antibody (HA601444) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse liver tissue lysate (negative)
    Lane 3: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 102 kDa
    Observed band size: 140 kDa

    Exposure time: 8 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601444) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"