MTAP Recombinant Mouse Monoclonal Antibody [PSH13-98]
Catalog# HA601445
MTAP Recombinant Mouse Monoclonal Antibody [PSH13-98]
-
WB
-
IF-Cell
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
MTAP Recombinant Mouse Monoclonal Antibody [PSH13-98]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human MTAP aa 1-283.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 31 kDa
Positive Control
HCT 116 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, NIH/3T3, C6.
Conjugation
unconjugated
Clone Number
PSH13-98
Product Features
Form
Liquid
Concentration
0.375 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IF-Cell
-
1:50
Target
Function
S-methyl-5'-thioadenosine phosphorylase (MTAP) is an enzyme responsible for polyamine metabolism. In humans, it is encoded by the methylthioadenosine phosphorylase (MTAP) gene on chromosome 9. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. It is responsible for the first step in this pathway, where it catalyzes the reversible phosphorylation of MTA to adenine and 5-methylthioribose-1-phosphate. This takes place after MTA is generated from S-adenosylmethionine.
Background References
1. Brune MM et al. MTAP as an emerging biomarker in thoracic malignancies. Lung Cancer. 2024 Nov
2. Patro CPK et al. MTAP loss: a possible therapeutic approach for glioblastoma. J Transl Med. 2022 Dec
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
5' methylthioadenosine phosphorylase antibody
5''-methylthioadenosine phosphorylase antibody
BDMF antibody
c86fus antibody
DMSFH antibody
DMSMFH antibody
Epididymis luminal protein 249 antibody
HEL 249 antibody
LGMBF antibody
MeSAdo phosphorylase antibody
ExpandImages
-
☑ Relative expression (RE)
Western blot analysis of MTAP on different lysates with Mouse anti-MTAP antibody (HA601445) at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 2: HeLa cell lysate
Lane 3: A549 cell lysate (negative)
Lane 4: NIH/3T3 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601445) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of NIH/3T3 cells labeling MTAP with Mouse anti-MTAP antibody (HA601445) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MTAP antibody (HA601445) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C6 cells labeling MTAP with Mouse anti-MTAP antibody (HA601445) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MTAP antibody (HA601445) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"