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NeuN Recombinant Antibody [SR45-07] - Guinea pig IgG2 (Chimeric)

Usd: 350

Specification

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Catalog# HA601481

NeuN Recombinant Antibody [SR45-07] - Guinea pig IgG2 (Chimeric)

Application

  • IHC-Fr

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

NeuN Recombinant Antibody [SR45-07] - Guinea pig IgG2 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within human NeuN aa 20-60.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 34 kDa

Positive Control

Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

SR45-07

Reactivity Data

IHC-Fr WB IHC-P IF-Tissue
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:1,000

  • WB

  • 1:10,000

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:500

Target

Function

Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.

Background References

1. Santamaría G et al. NeuN distribution in brain structures of normal and Zika-infected suckling mice. J Mol Histol. 2023 Jun

2. Luijerink L et al. Immunostaining for NeuN Does Not Show all Mature and Healthy Neurons in the Human and Pig Brain: Focus on the Hippocampus. Appl Immunohistochem Mol Morphol. 2021 Jul

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q8BIF2 Mouse

Unigene: 143966 Rat

Synonyms

FLJ56884 antibody

FLJ58356 antibody

Fox-1 homolog C antibody

fox1 homolog C antibody

Fox3 antibody

FOX3NeuN antibody

hexaribonucleotide binding protein 3 antibody

HRNBP3 antibody

NEUN antibody

neuronal nuclei antibody

Expand

Images

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Cerebral cortex<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× PBST<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601481" style="font-weight: bold;text-decoration: underline;">HA601481</a>, 1/1,000, overnight at 4℃.<br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Cerebral cortex
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× PBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA601481, 1/1,000, overnight at 4℃.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of NeuN on different lysates with Guinea pig anti-NeuN antibody (<a href="/products/HA601481" style="font-weight: bold;text-decoration: underline;">HA601481</a>) at 1/10,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: C2C12 cell lysate (negative)<br />Lane 3: Rat brain tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 34 kDa<br />Observed band size: 45/50 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601481" style="font-weight: bold;text-decoration: underline;">HA601481</a>) at 1/10,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (<a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of NeuN on different lysates with Guinea pig anti-NeuN antibody (HA601481) at 1/10,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: C2C12 cell lysate (negative)
    Lane 3: Rat brain tissue lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 34 kDa
    Observed band size: 45/50 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601481) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /><br />Species: Human<br />Tissue: Brain<br />Sample: Paraffin-embedded section <br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. <br /><br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601481" style="font-weight: bold;text-decoration: underline;">HA601481</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601481, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1021, 30 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /><br />Species: Mouse<br />Tissue: Brain<br />Sample: Paraffin-embedded section <br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. <br /><br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601481" style="font-weight: bold;text-decoration: underline;">HA601481</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601481, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1021, 30 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /><br />Species: Rat<br />Tissue: Brain<br />Sample: Paraffin-embedded section <br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. <br /><br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601481" style="font-weight: bold;text-decoration: underline;">HA601481</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601481, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1021, 30 minutes at room temperature.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Human<br />Tissue: Brain<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBST<br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601481" style="font-weight: bold;text-decoration: underline;">HA601481</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Human
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601481, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Mouse<br />Tissue: Brain<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBST<br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601481" style="font-weight: bold;text-decoration: underline;">HA601481</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601481, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Rat<br />Tissue: Brain<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBST<br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601481" style="font-weight: bold;text-decoration: underline;">HA601481</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Rat
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601481, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"