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pan Cytokeratin Recombinant Antibody [PDH09-10] - Rat IgG1 (Chimeric)

Usd: 350

Specification

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Catalog# HA601519

pan Cytokeratin Recombinant Antibody [PDH09-10] - Rat IgG1 (Chimeric)

Application

  • IHC-P

  • mIHC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

pan Cytokeratin Recombinant Antibody [PDH09-10] - Rat IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Full length native protein corresponding to Human pan Cytokeratin.

Species Reactivity

Human

Validated Applications

IHC-P, mIHC

Positive Control

Human stomach carcinoma tissue, human liver tissue.

Conjugation

unconjugated

Clone Number

PDH09-10

Reactivity Data

IHC-P mIHC
Human
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:2,000

  • mIHC

  • 1:2,000

Target

Function

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Since each epithelium contains at least one acidic and one basic keratin, this antibody is used to observe the distribution of keratin-containing cells in normal epithelia and to identify neoplasms derived from such epithelium. Recognizes humancytokeratins 10, 14, 15, 16 and 19, and cytokeratins 1, 2, 3, 4, 5, 6, 7 and 8.

Background References

暂无

Subcellular Location

Cytoplasmic

UNIPROT

SwissProt: Q01546 Human

SwissProt: Q7Z794 Human

SwissProt: P48668 Human

SwissProt: P13645 Human

Synonyms

pan Cytokeratin antibody

pan-Cytokeratin antibody

pan ck antibody

pan-ck antibody

panck antibody

pan Keratin antibody

Images

  • Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rat anti-pan Cytokeratin antibody (<a href="/products/HA601519" style="font-weight: bold;text-decoration: underline;">HA601519</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601519" style="font-weight: bold;text-decoration: underline;">HA601519</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rat anti-pan Cytokeratin antibody (HA601519) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601519) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rat anti-pan Cytokeratin antibody (<a href="/products/HA601519" style="font-weight: bold;text-decoration: underline;">HA601519</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601519" style="font-weight: bold;text-decoration: underline;">HA601519</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rat anti-pan Cytokeratin antibody (HA601519) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601519) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"