pan Cytokeratin Recombinant Antibody [PDH09-10] - Rat IgG1 (Chimeric)
Catalog# HA601519
pan Cytokeratin Recombinant Antibody [PDH09-10] - Rat IgG1 (Chimeric)
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IHC-P
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mIHC
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Human
-
unconjugated
Overview
Product Name
pan Cytokeratin Recombinant Antibody [PDH09-10] - Rat IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Full length native protein corresponding to Human pan Cytokeratin.
Species Reactivity
Human
Validated Applications
IHC-P, mIHC
Positive Control
Human stomach carcinoma tissue, human liver tissue.
Conjugation
unconjugated
Clone Number
PDH09-10
Reactivity Data
| IHC-P | mIHC | |
|---|---|---|
| Human |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-P
-
1:2,000
-
mIHC
-
1:2,000
Target
Function
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Since each epithelium contains at least one acidic and one basic keratin, this antibody is used to observe the distribution of keratin-containing cells in normal epithelia and to identify neoplasms derived from such epithelium. Recognizes humancytokeratins 10, 14, 15, 16 and 19, and cytokeratins 1, 2, 3, 4, 5, 6, 7 and 8.
Background References
暂无
Subcellular Location
Cytoplasmic
UNIPROT
Synonyms
pan Cytokeratin antibody
pan-Cytokeratin antibody
pan ck antibody
pan-ck antibody
panck antibody
pan Keratin antibody
Images
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Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rat anti-pan Cytokeratin antibody (HA601519) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601519) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rat anti-pan Cytokeratin antibody (HA601519) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601519) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"