TRBC1 Recombinant Mouse Monoclonal Antibody [PSH15-51]
Catalog# HA601524
TRBC1 Recombinant Mouse Monoclonal Antibody [PSH15-51]
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FC
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Human
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unconjugated
Overview
Product Name
TRBC1 Recombinant Mouse Monoclonal Antibody [PSH15-51]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Species Reactivity
Human
Validated Applications
FC
Target Molecular Weight
Predicted band size: 20 kDa
Positive Control
Human peripheral blood cells.
Conjugation
unconjugated
Clone Number
PSH15-51
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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FC
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5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Target
Function
Constant region of T cell receptor (TR) beta chain. Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens. Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity.
Background References
1. Nichakawade TD et al. TRBC1-targeting antibody-drug conjugates for the treatment of T cell cancers. Nature. 2024 Apr
2. Horna P et al. Emerging Role of T-cell Receptor Constant beta Chain-1 (TRBC1) Expression in the Flow Cytometric Diagnosis of T-cell Malignancies. Int J Mol Sci. 2021 Feb
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
T cell receptor beta constant 1
TRBC1
Images
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Flow cytometric analysis of human peripheral blood cells labeling TRBC1 (HA601524).
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601524, 1T). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Compared with Mouse IgG Isotype Control (iFluor™ 488, 1/1,000).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"