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PE Conjugated Human IgE Recombinant Mouse Monoclonal Antibody [PSH14-50]

Usd: 269

Specification

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Catalog# HA601527F

PE Conjugated Human IgE Recombinant Mouse Monoclonal Antibody [PSH14-50]

Application

  • FC

Reactivity

  • Human

Conjugation

  • PE

Overview

Product Name

PE Conjugated Human IgE Recombinant Mouse Monoclonal Antibody [PSH14-50]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Species Reactivity

Human

Validated Applications

FC

Molecular Weight

Predicted band size: 60 kDa

Positive Control

Human peripheral blood cells.

Conjugation

PE

Clone Number

PSH14-50

Reactivity Data

FC
Human
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at 2℃ to 8℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 1% BSA, 0.2% Proclean 950.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • FC

  • 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Target

Function

Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.

Background References

暂无

Subcellular Location

Secreted, Cell membrane.

UNIPROT

SwissProt: P0DOX4 Human

Images

  • Flow cytometric analysis of human peripheral blood cells labeling Human IgE (<a href="/products/HA601527F" style="font-weight: bold;text-decoration: underline;">HA601527F</a>, PE).<br /><br />Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with Human IgE (<a href="/products/HA601527F" style="font-weight: bold;text-decoration: underline;">HA601527F</a>, PE, 1/1,000) compared with Mouse IgG Isotype Control (PE, 1/1,000).

    Flow cytometric analysis of human peripheral blood cells labeling Human IgE (HA601527F, PE).

    Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with Human IgE (HA601527F, PE, 1/1,000) compared with Mouse IgG Isotype Control (PE, 1/1,000).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"