CD4 Recombinant Antibody [PSH08-22] - Mouse IgG1 (Chimeric)
Overview
Product Name
CD4 Recombinant Antibody [PSH08-22] - Mouse IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within mouse CD4 aa 27-394.
Species Reactivity
Mouse
Validated Applications
WB, IF-Tissue, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 51 kDa
Positive Control
Mouse spleen tissue lysate, Mouse thymus tissue lysate, mouse spleen tissue, mouse spleen cells.
Conjugation
unconjugated
Clone Number
PSH08-22
Reactivity Data
| WB | IF-Tissue | IHC-P | IF-Cell | |
|---|---|---|---|---|
| Mouse |
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|
|
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IF-Tissue
-
1:500
-
IHC-P
-
1:2,000
-
IF-Cell
-
1:100
Target
Function
In molecular biology, CD4 (cluster of differentiation 4) is a glycoprotein that serves as a co-receptor for the T-cell receptor (TCR). CD4 is found on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene. CD4+ T helper cells are white blood cells that are an essential part of the human immune system. They are often referred to as CD4 cells, T helper cells or T4 cells. They are called helper cells because one of their main roles is to send signals to other types of immune cells, including CD8 killer cells, which then destroy the infectious particle. If CD4 cells become depleted, for example in untreated HIV infection, or following immune suppression prior to a transplant, the body is left vulnerable to a wide range of infections that it would otherwise have been able to fight.
Background References
1. Ruterbusch M et al. In Vivo CD4(+) T Cell Differentiation and Function: Revisiting the Th1/Th2 Paradigm. Annu Rev Immunol. 2020 Apr
2. Oh DY et al. Cytotoxic CD4(+) T cells in cancer: Expanding the immune effector toolbox. Immunity. 2021 Dec
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD 4 antibody
CD4 (L3T4) antibody
CD4 antibody
CD4 antigen (p55) antibody
CD4 antigen antibody
CD4 molecule antibody
CD4 receptor antibody
CD4+ Lymphocyte deficiency, included antibody
CD4_HUMAN antibody
CD4mut antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD4 on different lysates with Mouse anti-CD4 antibody (HA601533) at 1/2,000 dilution.
Lane 1: Mouse spleen tissue lysate
Lane 2: C2C12 cell lysate (negative)
Lane 3: Mouse thymus tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 1 minute 34 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601533) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IF-Tissue
Species: Mouse
Site: spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD4 antibody (HA601533) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601533) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of mouse spleen cells labeling CD4 with Mouse anti-CD4 antibody (HA601533) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD4 antibody (HA601533) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Combination of LowDose Epigenetic Modifiers and TIC10 for the Activation of Antitumor Immunity and Inhibition of Tumor Growth in Gastrointestinal Cancer
Journal: Cancer Medicine
DOI: 10.1002/cam4.71061
IF: 3.1
Application: IHC
Reactivity: Mouse
Publish date: 2025 Jul