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CD11c Recombinant Antibody [SI19-06] - Mouse IgG1 (Chimeric)

Usd: 350

Specification

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Catalog# HA601545

CD11c Recombinant Antibody [SI19-06] - Mouse IgG1 (Chimeric)

Application

  • IF-Tissue

  • IHC-P

  • mIHC

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

CD11c Recombinant Antibody [SI19-06] - Mouse IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human CD11c aa 1,114-1,163 / 1,163 (Cytoplasmic).

Species Reactivity

Human

Validated Applications

IF-Tissue, IHC-P, mIHC

Molecular Weight

Predicted band size: 128 kDa

Positive Control

Human spleen tissue, human Hodgkin's lymphoma tissue, human tonsil tissue.

Conjugation

unconjugated

Clone Number

SI19-06

Reactivity Data

IF-Tissue IHC-P mIHC
Human
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Tissue

  • 1:500

  • IHC-P

  • 1:800

  • mIHC

  • 1:4,000

Target

Function

Integrin αX (CD11C, leukocyte surface antigen p150,95, CR4, Axb2) is a type 1 transmembrane protein that traditionally combines with β2 chain to form a leukocyte-specific integrin known as inactivated-C3b (iC3b) receptor 4 (CR4). Integrin αX/β2 shares similar properties of the αM/β2 integrin in mediating adherence of neutrophils and monocytes to stimulated endothelial cells, and in phagocytosis of complement coated particles. Abnormal expression of Integrin αX is characteristic of hairy cell leukemia (HCL) and is dependent upon activation of proto-oncogenes Ras and JunD. Proteins and DNA elements that influence transcription of Integrin αX include Sp1 and Sp1-like factors, AP-1 family, C/EBP, Oct-2 and PU.1. Integrin αX is present on monocyte derivative dendritic cells (DCs), macrophages and NK cells. Upon activation, DCs present in skin (Langerhans cells), lining of nose, lung, stomach, intestine and blood can migrate to lymphoid tissues and interact with T and B cells to initiate and shape the immune response.

Background References

1. Morandi F et al. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. J Immunol 192:2634-42 (2014).

2. Svensson MN et al. Fms-like tyrosine kinase 3 ligand controls formation of regulatory T cells in autoimmune arthritis. PLoS One 8:e54884 (2013).

Subcellular Location

Membrane.

UNIPROT

SwissProt: P20702 Human

Synonyms

95 alpha chain antibody

95 antibody

CD 11c antibody

CD11 antigen-like family member C antibody

CD11c antibody

CD11c antigen antibody

Complement component 3 receptor 4 subunit antibody

CR4 antibody

Integrin alpha X antibody

Integrin alpha X chain antibody

Expand

Images

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: spleen<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Human

    Site: spleen

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11c antibody (<a href="/products/HA601545" style="font-weight: bold;text-decoration: underline;">HA601545</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601545" style="font-weight: bold;text-decoration: underline;">HA601545</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11c antibody (HA601545) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601545) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue with Mouse anti-CD11c antibody (<a href="/products/HA601545" style="font-weight: bold;text-decoration: underline;">HA601545</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601545" style="font-weight: bold;text-decoration: underline;">HA601545</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue with Mouse anti-CD11c antibody (HA601545) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601545) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11c antibody (<a href="/products/HA601545" style="font-weight: bold;text-decoration: underline;">HA601545</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601545" style="font-weight: bold;text-decoration: underline;">HA601545</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11c antibody (HA601545) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601545) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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