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Calreticulin Recombinant Antibody [SU37-03] - Mouse IgG1 (Chimeric)

Usd: 350

Specification

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Catalog# HA601547

Calreticulin Recombinant Antibody [SU37-03] - Mouse IgG1 (Chimeric)

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Calreticulin Recombinant Antibody [SU37-03] - Mouse IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human Calreticulin aa 40-89 / 417.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 48 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human breast carcinoma tissue, human liver tissue, mouse liver tissue, rat liver tissue.

Conjugation

unconjugated

Clone Number

SU37-03

Reactivity Data

WB IHC-P IF-Cell
Human
Tested Tested
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Green Monkey
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:10,000

  • IHC-P

  • 1:20,000

  • IF-Cell

  • 1:1,000

Target

Function

Calnexin and calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor calregulin contains the calcium-binding "E-F hand" motif found in calmodulins. Calnexin and calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.

Background References

1. Kojima Y et al. Cyclin-dependent kinase inhibitor 2B regulates efferocytosis and atherosclerosis. J Clin Invest 124:1083-97 (2014).

2. Angelova AL et al. Complementary induction of immunogenic cell death by oncolytic parvovirus H-1PV and gemcitabine in pancreatic cancer. J Virol 88:5263-76 (2014).

Subcellular Location

Endoplasmic reticulum lumen, Cytoplasm, Secreted, Cell surface, Sarcoplasmic reticulum lumen, nucleus.

UNIPROT

SwissProt: P27797 Human

SwissProt: P14211 Mouse

SwissProt: P18418 Rat

Synonyms

Autoantigen RO antibody

CALR antibody

CALR protein antibody

CALR_HUMAN antibody

Calregulin antibody

Calreticulin antibody

cC1qR antibody

CRP55 antibody

CRT antibody

CRTC antibody

Expand

Images

  • Application: Immunocytochemistry (IF-cell)<br /><br />Species: Human<br />Sample: HeLa (Human cervix adenocarcinoma epithelial cell)<br /><br />Fixation: Ice cold 100% methanol, 5 minutes at room temperature.<br />Permeabilization: /<br /><br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.<br />Antibody dilution buffer: 1% BSA in PBST.<br />Primary antibody: <a href="/products/HA601547" style="font-weight: bold;text-decoration: underline;">HA601547</a>, 1/1,000, overnight at 4℃.<br />Secondary antibody: Goat Anti-Mouse IgG (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>), 45 minutes at room temperature.<br /><br />Counterstain: Beta tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, Red), 1/200, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: HeLa (Human cervix adenocarcinoma epithelial cell)

    Fixation: Ice cold 100% methanol, 5 minutes at room temperature.
    Permeabilization: /

    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.
    Primary antibody: HA601547, 1/1,000, overnight at 4℃.
    Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.

    Counterstain: Beta tubulin (ET1602-4, Red), 1/200, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

  • Western blot analysis of Calreticulin on different lysates with Mouse anti-Calreticulin antibody (<a href="/products/HA601547" style="font-weight: bold;text-decoration: underline;">HA601547</a>) at 1/10,000 dilution.<br /><br />Lane 1: HepG2 cell lysate <br />Lane 2: HeLa cell lysate <br />Lane 3: C2C12 cell lysate <br />Lane 4: C6 cell lysate <br />Lane 5: COS-1 cell lysate <br />Lane 6: Mouse liver tissue lysate<br />Lane 7: Rat liver tissue lysate <br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 48 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601547" style="font-weight: bold;text-decoration: underline;">HA601547</a>) at 1/10,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Calreticulin on different lysates with Mouse anti-Calreticulin antibody (HA601547) at 1/10,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: C2C12 cell lysate
    Lane 4: C6 cell lysate
    Lane 5: COS-1 cell lysate
    Lane 6: Mouse liver tissue lysate
    Lane 7: Rat liver tissue lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 48 kDa
    Observed band size: 55 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601547) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Calreticulin antibody (<a href="/products/HA601547" style="font-weight: bold;text-decoration: underline;">HA601547</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601547" style="font-weight: bold;text-decoration: underline;">HA601547</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Calreticulin antibody (HA601547) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601547) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Calreticulin antibody (<a href="/products/HA601547" style="font-weight: bold;text-decoration: underline;">HA601547</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601547" style="font-weight: bold;text-decoration: underline;">HA601547</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Calreticulin antibody (HA601547) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601547) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-Calreticulin antibody (<a href="/products/HA601547" style="font-weight: bold;text-decoration: underline;">HA601547</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601547" style="font-weight: bold;text-decoration: underline;">HA601547</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-Calreticulin antibody (HA601547) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601547) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-Calreticulin antibody (<a href="/products/HA601547" style="font-weight: bold;text-decoration: underline;">HA601547</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601547" style="font-weight: bold;text-decoration: underline;">HA601547</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-Calreticulin antibody (HA601547) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601547) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Citation