Skip to content

CD68 Recombinant Antibody [PSH06-65] - Guinea pig IgG2 (Chimeric)

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA601595

CD68 Recombinant Antibody [PSH06-65] - Guinea pig IgG2 (Chimeric)

Application

  • WB

  • IHC-Fr

  • IHC-P

  • IF-Tissue

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

CD68 Recombinant Antibody [PSH06-65] - Guinea pig IgG2 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within mouse CD68 aa 1-316.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-Fr, IHC-P, IF-Tissue

Target Molecular Weight

Predicted band size: 35 kDa

Positive Control

RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate.

Conjugation

unconjugated

Clone Number

PSH06-65

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-Fr

  • 1:200

  • IHC-P

  • 1:2,000

  • IF-Tissue

  • 1:200

Target

Function

CD68 (Cluster of Differentiation 68) is a protein highly expressed by cells in the monocyte lineage (e.g., monocytic phagocytes, osteoclasts), by circulating macrophages, and by tissue macrophages (e.g., Kupffer cells, microglia). Other names or aliases for this gene in humans and other animals include: CD68 Molecule, CD68 Antigen, GP110, Macrosialin, Scavenger Receptor Class D, Member 1, SCARD1, and LAMP4. The mouse equivalent is known as "macrosialin".

Background References

1. Choudhary M et al. CD68: Potential Contributor to Inflammation and RPE Cell Dystrophy. Adv Exp Med Biol. 2023

2. Deng R et al. Periosteal CD68(+) F4/80(+) Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-beta1. Adv Sci (Weinh). 2022 Jan

Subcellular Location

Endosome membrane, Lysosome membrane; Cell membrane.

UNIPROT

SwissProt: P31996 Mouse

Entrez Gene: 287435 Rat

Synonyms

CD 68 antibody

CD68 antibody

CD68 antigen antibody

CD68 molecule antibody

CD68_HUMAN antibody

DKFZp686M18236 antibody

gp11 antibody

Gp110 antibody

LAMP4 antibody

Macrophage antigen CD68 (microsialin) antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of CD68 on different lysates with Guinea pig anti-CD68 antibody (<a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>) at 1/2,000 dilution.<br /><br />Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate (10 µg/Lane)<br />Lane 2: RAW264.7 treated with PNGase F cell lysate (10 µg/Lane)<br />Lane 3: Mouse spleen tissue lysate (20 µg/Lane)<br />Lane 4: Rat spleen tissue lysate (20 µg/Lane)<br /><br />Exposure time: 20 seconds; ECL: K1801<br /><br />CD68 is a glycoprotein and can be deglycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID : 7680921)<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Rabbit anti-Guinea pig IgG-HRP (<a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 35 kDa<br />Observed band size: 60-100 kDa

    ☑ Cell treatment (CT)

    Western blot analysis of CD68 on different lysates with Guinea pig anti-CD68 antibody (HA601595) at 1/2,000 dilution.

    Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate (10 µg/Lane)
    Lane 2: RAW264.7 treated with PNGase F cell lysate (10 µg/Lane)
    Lane 3: Mouse spleen tissue lysate (20 µg/Lane)
    Lane 4: Rat spleen tissue lysate (20 µg/Lane)

    Exposure time: 20 seconds; ECL: K1801

    CD68 is a glycoprotein and can be deglycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID : 7680921)

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601595, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Rabbit anti-Guinea pig IgG-HRP (HA1021), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 35 kDa
    Observed band size: 60-100 kDa

  • Application: Immunofluorescence (IHC-Fr) <br /> <br />Species: Mouse <br />Tissue: Spleen <br />Sample: Frozen section <br /> <br />Antigen retrieval: Not required <br /> <br />Wash buffer: 1× PBST <br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Spleen
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× PBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr) <br /> <br />Species: Rat <br />Tissue: Spleen <br />Sample: Frozen section <br /> <br />Antigen retrieval: Not required <br /> <br />Wash buffer: 1× PBST <br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Rat
    Tissue: Spleen
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× PBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Mouse<br />Tissue: Liver<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/2,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1021, 30 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Mouse<br />Tissue: Spleen<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/2,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1021, 30 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Rat<br />Tissue: Liver<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/2,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1021, 30 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P)<br /><br />Species: Rat<br />Tissue: Spleen<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/2,000, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1021, 30 minutes at room temperature.

  • Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Mouse <br />Tissue: Spleen <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× PBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Rat <br />Tissue: Spleen <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× PBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601595" style="font-weight: bold;text-decoration: underline;">HA601595</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Rat
    Tissue: Spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× PBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601595, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"