FOLR1 Recombinant Mouse Monoclonal Antibody [PSH23-62]
Catalog# HA601607
FOLR1 Recombinant Mouse Monoclonal Antibody [PSH23-62]
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WB
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IF-Cell
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FC
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Human
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HA610407
不含抗保成分
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unconjugated
Overview
Product Name
FOLR1 Recombinant Mouse Monoclonal Antibody [PSH23-62]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 30 kDa
Positive Control
JAR (Human placental choriocarcinoma cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate.
Conjugation
unconjugated
Clone Number
PSH23-62
Product Features
Form
Liquid
Concentration
0.2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:4,000
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IF-Cell
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1:100
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FC
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1:200
Target
Function
Folate receptor 1 (Folate receptor alpha, FOLR1) is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family. Members of this family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This receptor is responsible for binding to folic acid and its derivatives, which becomes crucial during fetal development. By adding folate supplementation during pregnancy, neural tube defects in the fetus are prevented. Folate derivatives are necessary for important metabolic processes such as DNA, protein and lipid methylation. More importantly, folate plays a major role in DNA replication and cell division, which are common characteristics of rapid growth. Even though it is unclear how folate affects neural tube formation, scientists are certain that without appropriate folate levels, neural tube defects can develop through human and mice studies. Neural tube defects refer to the improper development of the neural tube by not being sealed correctly. This results in exencephaly or spina bifida, both nervous system abnormalities. This gene is composed of 7 exons; exons 1 through 4 encode the 5' UTR and exons 4 through 7 encode the open reading frame. Due to the presence of 2 promoters, multiple transcription start sites, and alternative splicing of exons, several transcript variants are derived from this gene. These variants differ in the lengths of 5' and 3' UTR, but they encode an identical amino acid sequence.
Background References
1. Nawaz FZ et al. Emerging roles for folate receptor FOLR1 in signaling and cancer. Trends Endocrinol Metab. 2022 Mar
2. Liang Z et al. Tandem CAR-T cells targeting FOLR1 and MSLN enhance the antitumor effects in ovarian cancer. Int J Biol Sci. 2021 Oct
Subcellular Location
Cell membrane, Apical cell membrane, Basolateral cell membrane, Secreted, Cytoplasmic vesicle, clathrin-coated vesicle, Endosome.
UNIPROT
Synonyms
adult antibody
Adult folate binding protein antibody
Adult folate-binding protein antibody
FBP antibody
Folate Binding Protein antibody
Folate Receptor 1 Adult antibody
Folate receptor 1 antibody
Folate Receptor 1 Precursor antibody
Folate receptor adult antibody
Folate receptor alpha antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of FOLR1 on different lysates with Mouse anti-FOLR1 antibody (HA601607) at 1/4,000 dilution.
Lane 1: JAR (Human placental choriocarcinoma cells) cell lysate
Lane 2: K-562 (Human chronic myelogenous leukemia cells) cell lysate
Lane 3: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 6 seconds; ECL: K1801
Negative control : K-562 (PMID : 15899836).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601607, 1/4,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 30 kDa
Observed band size: 38 kDa -
☑ Relative expression (RE)
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: HeLa (Human cervix adenocarcinoma epithelial cell)/K-562 (Human chronic myelogenous leukemia cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA601607, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, red), 1/200, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
Negative control : K-562 (PMID : 15899836). -
☑ Relative expression (RE)
Application: Flow Cytometry (Intra)
Species: Human
Sample: HeLa (Human cervix adenocarcinoma epithelial cell)/K-562 (Human chronic myelogenous leukemia cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA601607 (1/200) (Red) compared with Mouse IgG1 Isotype Control (HA601336, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 15 minutes at room temperature.
Negative control : K-562 (PMID : 15899836).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"