Rabbit IgG heavy chain(Fc fragment) Recombinant Mouse Monoclonal Antibody [PSH23-92] - BSA and Azide free
Catalog# HA601651
Rabbit IgG heavy chain(Fc fragment) Recombinant Mouse Monoclonal Antibody [PSH23-92] - BSA and Azide free
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WB
-
ELISA
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Rabbit
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unconjugated
Overview
Product Name
Rabbit IgG heavy chain(Fc fragment) Recombinant Mouse Monoclonal Antibody [PSH23-92] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Purified Rabbit IgG heavy chain(Fc fragment).
Species Reactivity
Rabbit
Validated Applications
WB, ELISA
Target Molecular Weight
50 kDa
Conjugation
unconjugated
Clone Number
PSH23-92
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | Elisa | |
|---|---|---|
| rabbit |
|
|
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
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ELISA
-
1:5,000
Target
Function
The fundamental structure of IgG contains two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa, thus tetrameric quaternary structure. Light chain, which is usually same in a natural IgG molecule, includes κ chain and λ chain, but κ or λ antibody can exist in one body simultaneously. A heavy chain includes Fab- and Fc-Fragments. This antibody can recognize rabbit IgG Fc fragments of heavy chains.
Background References
暂无
Subcellular Location
secrete
Images
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Western blot analysis of Rabbit IgG heavy chain(Fc fragment) on Rabbit IgG (positive) with Mouse anti-Rabbit IgG heavy chain(Fc fragment) antibody (HA601651) at 1/50,000 dilution.
Lysates/proteins at 10 µg/Lane.
Exposure time: 1 minute; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601651, 1/50,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Observed band size: 50 kDa -
☑ Relative expression (RE)
Western blot analysis of Rabbit IgG heavy chain(Fc fragment) on Mouse IgG (negative) with Mouse anti-Rabbit IgG heavy chain(Fc fragment) antibody (HA601651) at 1/50,000 dilution.
Lysates/proteins at 10 µg/Lane.
Exposure time: 1 minute; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601651, 1/50,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature -
☑ Relative expression (RE)
Western blot analysis of Rabbit IgG heavy chain(Fc fragment) on Human IgG (negative) with Mouse anti-Rabbit IgG heavy chain(Fc fragment) antibody (HA601651) at 1/50,000 dilution.
Lysates/proteins at 10 µg/Lane.
Exposure time: 1 minute; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601651, 1/50,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature -
Indirect ELISA analysis of Rabbit IgG heavy chain(Fc fragment) was performed by coating wells of a 96-well plate with 50 µL per well of Fc fragment of Rabbit lgG / F(ab')₂ fragment of Rabbit IgG / Human IgG diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer for 1 hour at 37℃, and incubated with 50 µL per well of Rabbit IgG heavy chain(Fc fragment) recombinant Mouse monoclonal antibody serial diluted starting from a concentration of 4µg/mL for 45 minutes at 37℃. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1/80,000 for 30 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"