Cathepsin D Recombinant Mouse Monoclonal Antibody [13F3-R] - BSA and Azide free
Catalog# HA610001
Cathepsin D Recombinant Mouse Monoclonal Antibody [13F3-R] - BSA and Azide free
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WB
-
IHC-P
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Human
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EM1901-02
含抗保成分
-
unconjugated
Overview
Product Name
Cathepsin D Recombinant Mouse Monoclonal Antibody [13F3-R] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human Cathepsin D aa 1-432.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 45 kDa
Positive Control
MCF7 cell lysate, U-937 cell lysate, SK-Br-3 cell lysate, HepG2 cell lysate, A431 cell lysate, Human liver tissue lysate, human liver tissue, human liver cancer tissue, human breast cancer tissue.
Conjugation
unconjugated
Clone Number
13F3-R
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:2,000-1:5,000
Target
Function
The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsins may also cleave some protein precursors, thereby releasing regulatory peptides. The promoter region of the cathepsin D gene contains five Sp1 binding sites and four AP-2 binding sites. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease.
Background References
1. Sadleir KR et al. Presynaptic dystrophic neurites surrounding amyloid plaques are sites of microtubule disruption, BACE1 elevation, and increased Aß generation in Alzheimer\'s disease. Acta Neuropathol 132:235-56 (2016).
2. Santaguida S et al. Aneuploidy-induced cellular stresses limit autophagic degradation. Genes Dev 29:2010-21 (2015).
Sequence Similarity
Belongs to the peptidase A1 family.
Tissue Specificity
Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level).
Post-translational Modification
N- and O-glycosylated.; Undergoes proteolytic cleavage and activation by ADAM30.; As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified.
Subcellular Location
Lysosome. Melanosome. Secreted, extracellular space.
UNIPROT
Synonyms
CatD antibody
CATD_HUMAN antibody
Cathepsin D antibody
Cathepsin D heavy chain antibody
CD antibody
Ceroid lipofuscinosis neuronal 10 antibody
CLN10 antibody
CPSD antibody
ctsd antibody
Epididymis secretory sperm binding protein Li 130P antibody
ExpandImages
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Western blot analysis of Cathepsin D on different lysates with Mouse anti-Cathepsin D antibody (HA610001) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: U-937 cell lysate (20 µg/Lane)
Lane 3: SK-Br-3 cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: A431 cell lysate (20 µg/Lane)
Lane 6: Human liver tissue lysate (40 µg/Lane)
Predicted band size: 45 kDa
Observed band size: 45/28 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-02) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Cathepsin D antibody (HA610001) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-02) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-Cathepsin D antibody (HA610001) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-02) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-Cathepsin D antibody (HA610001) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-02) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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