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QKI-5 Recombinant Mouse Monoclonal Antibody [PSH10-22] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610223

QKI-5 Recombinant Mouse Monoclonal Antibody [PSH10-22] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

Overview

Product Name

QKI-5 Recombinant Mouse Monoclonal Antibody [PSH10-22] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within human QKI-5 aa 301-341 / 341.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IHC-Fr, IP

Target Molecular Weight

Predicted band size: 38 kDa

Positive Control

K-562 cell lysate, HeLa cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, mouse cerebellum tissue, rat cerebellum tissue.

Conjugation

unconjugated

Clone Number

PSH10-22

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:5,000-1:20,000

  • IHC-Fr

  • 1:500

  • IP

  • 1-2μg/sample

Target

Function

Quaking homolog, KH domain RNA binding (mouse), also known as QKI, is a protein which in humans is encoded by the QKI gene. QKI belongs to a family of RNA-binding proteins called STAR proteins for Signal Transduction and Activation of RNA. They have an HNRNPK homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1) and SF1. Two more new members are KHDRBS3 and KHDRBS2. The QKI gene is implicated as being important in schizophrenia, and QKI controls translation of many oligodendrocyte-related genes.

Background References

1. Zhao Z et al. QKI shuttles internal m(7)G-modified transcripts into stress granules and modulates mRNA metabolism. Cell. 2023 Jul

2. Suh YY et al. MYB/MYBL1::QKI fusion-positive diffuse glioma. J Neuropathol Exp Neurol. 2023 Feb

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q96PU8-1 Human

SwissProt: Q9QYS9 Mouse

SwissProt: Q91XU1 Rat

Synonyms

DKFZp586I0923 antibody

HKQ antibody

Homolog of mouse quaking QKI KH domain RNA binding protein antibody

Hqk antibody

HQK1 antibody

HqkI antibody

OTTHUMP00000017581 antibody

OTTHUMP00000017582 antibody

OTTHUMP00000017583 antibody

Protein quaking antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • Western blot analysis of QKI-5 on different lysates with Mouse anti-QKI-5 antibody (<a href="/products/HA610223" style="font-weight: bold;text-decoration: underline;">HA610223</a>) at 1/2,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: Mouse brain tissue lysate<br />Lane 4: Mouse cerebellum tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610223" style="font-weight: bold;text-decoration: underline;">HA610223</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of QKI-5 on different lysates with Mouse anti-QKI-5 antibody (HA610223) at 1/2,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: Mouse brain tissue lysate
    Lane 4: Mouse cerebellum tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610223) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-QKI-5 antibody (<a href="/products/HA610223" style="font-weight: bold;text-decoration: underline;">HA610223</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610223" style="font-weight: bold;text-decoration: underline;">HA610223</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-QKI-5 antibody (HA610223) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610223) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-QKI-5 antibody (<a href="/products/HA610223" style="font-weight: bold;text-decoration: underline;">HA610223</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610223" style="font-weight: bold;text-decoration: underline;">HA610223</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-QKI-5 antibody (HA610223) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610223) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Indirect ELISA analysis of QKI-5 on different peptides.

    Indirect ELISA analysis of QKI-5 on different peptides.

  • QKI-5 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA610223" style="font-weight: bold;text-decoration: underline;">HA610223</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA610223" style="font-weight: bold;text-decoration: underline;">HA610223</a> at 1/1,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA610223" style="font-weight: bold;text-decoration: underline;">HA610223</a> IP in HeLa cell lysate<br />Lane 3: Mouse IgG instead of <a href="/products/HA610223" style="font-weight: bold;text-decoration: underline;">HA610223</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 1 minute 7 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    QKI-5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA610223 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA610223 at 1/1,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA610223 IP in HeLa cell lysate
    Lane 3: Mouse IgG instead of HA610223 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 1 minute 7 seconds; ECL: K1801

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-QKI-5 antibody (<a href="/products/HA610223" style="font-weight: bold;text-decoration: underline;">HA610223</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610223" style="font-weight: bold;text-decoration: underline;">HA610223</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-QKI-5 antibody (HA610223) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610223) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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