QKI-5 Recombinant Mouse Monoclonal Antibody [PSH10-22]
Catalog# HA601378
QKI-5 Recombinant Mouse Monoclonal Antibody [PSH10-22]
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WB
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IHC-P
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IHC-Fr
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IP
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
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unconjugated
Overview
Product Name
QKI-5 Recombinant Mouse Monoclonal Antibody [PSH10-22]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human QKI-5 aa 301-341 / 341.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IHC-Fr, IP
Target Molecular Weight
Predicted band size: 38 kDa
Positive Control
K-562 cell lysate, HeLa cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, mouse cerebellum tissue, rat cerebellum tissue.
Conjugation
unconjugated
Clone Number
PSH10-22
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IHC-Fr | IF-Cell | IP | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:5,000-1:20,000
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IHC-Fr
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1:500
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IP
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1-2μg/sample
Target
Function
Quaking homolog, KH domain RNA binding (mouse), also known as QKI, is a protein which in humans is encoded by the QKI gene. QKI belongs to a family of RNA-binding proteins called STAR proteins for Signal Transduction and Activation of RNA. They have an HNRNPK homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1) and SF1. Two more new members are KHDRBS3 and KHDRBS2. The QKI gene is implicated as being important in schizophrenia, and QKI controls translation of many oligodendrocyte-related genes.
Background References
1. Zhao Z et al. QKI shuttles internal m(7)G-modified transcripts into stress granules and modulates mRNA metabolism. Cell. 2023 Jul
2. Suh YY et al. MYB/MYBL1::QKI fusion-positive diffuse glioma. J Neuropathol Exp Neurol. 2023 Feb
Subcellular Location
Nucleus, Cytoplasm.
Synonyms
DKFZp586I0923 antibody
HKQ antibody
Homolog of mouse quaking QKI KH domain RNA binding protein antibody
Hqk antibody
HQK1 antibody
HqkI antibody
OTTHUMP00000017581 antibody
OTTHUMP00000017582 antibody
OTTHUMP00000017583 antibody
Protein quaking antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Western blot analysis of QKI-5 on different lysates with Mouse anti-QKI-5 antibody (HA601378) at 1/2,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse cerebellum tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601378) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-QKI-5 antibody (HA601378) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601378) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-QKI-5 antibody (HA601378) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Indirect ELISA analysis of QKI-5 on different peptides.
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QKI-5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA601378 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601378 at 1/1,000 dilution. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA601378 IP in HeLa cell lysate
Lane 3: Mouse IgG instead of HA601378 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 7 seconds; ECL: K1801 -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-QKI-5 antibody (HA601378) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601378) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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