GABRA1 Recombinant Mouse Monoclonal Antibody [PSH10-46] - BSA and Azide free
Catalog# HA610227
GABRA1 Recombinant Mouse Monoclonal Antibody [PSH10-46] - BSA and Azide free
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WB
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Mouse
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Rat
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unconjugated
Overview
Product Name
GABRA1 Recombinant Mouse Monoclonal Antibody [PSH10-46] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human GABRA1 aa 334-421.
Species Reactivity
Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 52 kDa
Positive Control
Mouse cerebellum tissue lysate, Mouse brain tissue lysate, Rat cerebellum tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH10-46
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
Gamma-aminobutyric acid receptor subunit alpha-1 is a protein that in humans is encoded by the GABRA1 gene. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. The GABRA1 receptor is the specific target of the z-drug class of nonbenzodiazepine hypnotic agents and is responsible for their hypnotic and hallucinogenic effects.
Background References
1. Musto E et al. GABRA1-Related Disorders: From Genetic to Functional Pathways. Ann Neurol. 2023 Aug
2. Zeng Y et al. GABRA1 and GABRB2 Polymorphisms are Associated with Propofol Susceptibility. Pharmgenomics Pers Med. 2022 Feb
Subcellular Location
Postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane.
Synonyms
ECA4 antibody
EIEE19 antibody
EJM antibody
EJM5 antibody
Gaba receptor alpha 1 polypeptide antibody
GABA(A) receptor antibody
GABA(A) receptor subunit alpha 1 antibody
GABA(A) receptor subunit alpha-1 antibody
GABA(A) receptor, alpha 1 antibody
GABRA 1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of GABRA1 on different lysates with Mouse anti-GABRA1 antibody (HA610227) at 1/2,000 dilution.
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Mouse lung tissue lysate (negative)
Lane 4: Rat cerebellum tissue lysate
Lane 5: Rat brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610227) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"