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GABRA1 Recombinant Mouse Monoclonal Antibody [PSH10-46]

Usd: 350

Specification

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Catalog# HA601383

GABRA1 Recombinant Mouse Monoclonal Antibody [PSH10-46]

Application

  • WB

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

GABRA1 Recombinant Mouse Monoclonal Antibody [PSH10-46]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human GABRA1 aa 334-421.

Species Reactivity

Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 52 kDa

Positive Control

Mouse cerebellum tissue lysate, Mouse brain tissue lysate, Rat cerebellum tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-46

Reactivity Data

WB
Mouse
Tested Tested
Rat
Tested Tested
Human
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Gamma-aminobutyric acid receptor subunit alpha-1 is a protein that in humans is encoded by the GABRA1 gene. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. The GABRA1 receptor is the specific target of the z-drug class of nonbenzodiazepine hypnotic agents and is responsible for their hypnotic and hallucinogenic effects.

Background References

1. Musto E et al. GABRA1-Related Disorders: From Genetic to Functional Pathways. Ann Neurol. 2023 Aug

2. Zeng Y et al. GABRA1 and GABRB2 Polymorphisms are Associated with Propofol Susceptibility. Pharmgenomics Pers Med. 2022 Feb

Subcellular Location

Postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane.

UNIPROT

SwissProt: P62812 Mouse

SwissProt: P62813 Rat

Synonyms

ECA4 antibody

EIEE19 antibody

EJM antibody

EJM5 antibody

Gaba receptor alpha 1 polypeptide antibody

GABA(A) receptor antibody

GABA(A) receptor subunit alpha 1 antibody

GABA(A) receptor subunit alpha-1 antibody

GABA(A) receptor, alpha 1 antibody

GABRA 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of GABRA1 on different lysates with Mouse anti-GABRA1 antibody (<a href="/products/HA601383" style="font-weight: bold;text-decoration: underline;">HA601383</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse cerebellum tissue lysate<br />Lane 2: Mouse brain tissue lysate<br />Lane 3: Mouse lung tissue lysate (negative)<br />Lane 4: Rat cerebellum tissue lysate<br />Lane 5: Rat brain tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 52 kDa<br />Observed band size: 52 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601383" style="font-weight: bold;text-decoration: underline;">HA601383</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of GABRA1 on different lysates with Mouse anti-GABRA1 antibody (HA601383) at 1/2,000 dilution.

    Lane 1: Mouse cerebellum tissue lysate
    Lane 2: Mouse brain tissue lysate
    Lane 3: Mouse lung tissue lysate (negative)
    Lane 4: Rat cerebellum tissue lysate
    Lane 5: Rat brain tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601383) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"