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Mouse CD45R Recombinant Rat Monoclonal Antibody [PSH10-80] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610229

Mouse CD45R Recombinant Rat Monoclonal Antibody [PSH10-80] - BSA and Azide free

Application

  • IHC-P

  • FC

  • IF-Tissue

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Mouse CD45R Recombinant Rat Monoclonal Antibody [PSH10-80] - BSA and Azide free

Antibody Type

Recombinant Rat Monoclonal Antibody

Species Reactivity

Mouse

Validated Applications

IHC-P, FC, IF-Tissue

Target Molecular Weight

Predicted band size: 145 kDa

Positive Control

Mouse spleen tissue, mouse spleen cells.

Conjugation

unconjugated

Clone Number

PSH10-80

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:1,000

  • FC

  • 1:1,000

  • IF-Tissue

  • 1:500

Target

Function

Protein tyrosine phosphatase, receptor type, C also known as PTPRC is an enzyme that, in humans, is encoded by the PTPRC gene. PTPRC is also known as CD45 antigen (CD stands for cluster of differentiation), which was originally called leukocyte common antigen (LCA). PTPRC is a critical enzyme involved in regulating immune cell function. PTPRC is a transmembrane protein tyrosine phosphatase expressed on the surface of all nucleated hematopoietic cells, particularly lymphocytes. It plays a key role in the activation and differentiation of T cells, B cells, and other immune cells by modulating signaling pathways. It functions by dephosphorylating specific tyrosine residues on target proteins, thereby controlling various signaling processes essential for immune response and homeostasis.

Background References

1. Albekairi TH et al. Cadmium exposure exacerbates immunological abnormalities in a BTBR T(+) Itpr3(tf)/J autistic mouse model by upregulating inflammatory mediators in CD45R-expressing cells. J Neuroimmunol. 2024 Jan

2. Farombi EO et al. Kolaviron ameliorates behavioural deficit and injury to striatal dopaminergic terminals via modulation of oxidative burden, DJ-1 depletion and CD45R(+) cells infiltration in MPTP-model of Parkinson\'s disease. Metab Brain Dis. 2020 Aug

Subcellular Location

Cell membrane, Membrane raft, Synapse.

UNIPROT

SwissProt: P06800 Mouse

Synonyms

B220 antibody

CD45 antibody

CD45 antigen antibody

Glycoprotein antibody

GP180 antibody

L CA antibody

L-CA antibody

LCA antibody

Leukocyte common antigen antibody

Leukocyte common antigen precursor antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rat anti-Mouse CD45R antibody (<a href="/products/HA610229" style="font-weight: bold;text-decoration: underline;">HA610229</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610229" style="font-weight: bold;text-decoration: underline;">HA610229</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rat anti-Mouse CD45R antibody (HA610229) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610229) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of mouse spleen cells labeling Mouse CD45R (<a href="/products/HA610229" style="font-weight: bold;text-decoration: underline;">HA610229</a>).<br /><br />Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (<a href="/products/HA610229" style="font-weight: bold;text-decoration: underline;">HA610229</a>, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Dylight 488 conjugate-Goat anti-Rat IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.

    Flow cytometric analysis of mouse spleen cells labeling Mouse CD45R (HA610229).

    Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA610229, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Dylight 488 conjugate-Goat anti-Rat IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: spleen<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Mouse

    Site: spleen

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"