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Otx2 Recombinant Antibody [PSH01-61] - Rat IgG1 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610239

Otx2 Recombinant Antibody [PSH01-61] - Rat IgG1 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

Overview

Product Name

Otx2 Recombinant Antibody [PSH01-61] - Rat IgG1 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within human Otx2 aa 1-289.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

IHC-Fr, IHC-P

Target Molecular Weight

Predicted band size: 32 kDa

Positive Control

Mouse embryo tissue, rat embryo tissue.

Conjugation

unconjugated

Clone Number

PSH01-61

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:1,000-1:2,000

Target

Function

Homeobox protein OTX1 is a protein that in humans is encoded by the OTX1 gene. This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. The Otx gene is active in the region of the first gill arch, which is related to the upper and lower jaw and two of the bones of the ear. A similar protein in mice is required for proper brain and sensory organ development and can cause epilepsy.

Background References

1. Ibad RT et al. OTX2 stimulates adult retinal ganglion cell regeneration. Neural Regen Res. 2022 Mar

2. Du J et al. MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration. Signal Transduct Target Ther. 2023 Sep

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P32243 Human

SwissProt: P80206 Mouse

SwissProt: Q64201 Rat

Synonyms

CPHD6 antibody

Homeobox protein OTX2 antibody

MCOPS 5 antibody

MCOPS5 antibody

MGC45000 antibody

Orthodenticle 2 antibody

Orthodenticle homeobox 2 antibody

Orthodenticle homolog 2 (Drosophila) antibody

Orthodenticle homolog 2 antibody

Orthodenticle2 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: E14.5 embryo <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: E14.5 embryo

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rat anti-Otx2 antibody (<a href="/products/HA610239" style="font-weight: bold;text-decoration: underline;">HA610239</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610239" style="font-weight: bold;text-decoration: underline;">HA610239</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rat anti-Otx2 antibody (HA610239) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610239) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rat anti-Otx2 antibody (<a href="/products/HA610239" style="font-weight: bold;text-decoration: underline;">HA610239</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610239" style="font-weight: bold;text-decoration: underline;">HA610239</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rat anti-Otx2 antibody (HA610239) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610239) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rat anti-Otx2 antibody (<a href="/products/HA610239" style="font-weight: bold;text-decoration: underline;">HA610239</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610239" style="font-weight: bold;text-decoration: underline;">HA610239</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rat anti-Otx2 antibody (HA610239) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610239) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"